Simple Plex Mouse IL-5 Cartridge

Name: Simple Plex Mouse IL-5 Cartridge
Brand: ProteinSimple
SKU: SPCKB-MP-002846

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-MP-002846

Product Datasheet / COA

Simple Plex Mouse IL-5 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Mouse Cell Culture Supernates (10 uL), Mouse Serum (25 uL), Mouse EDTA Plasma (25 uL), Mouse Heparin Plasma (25 uL)
Sensitivity:	0.17 pg/mL
Assay Range:	0.45 - 4,290 pg/mL (Mouse Cell Culture Supernates, Mouse Serum, Mouse EDTA Plasma, Mouse Heparin Plasma)
Simple Plex Mouse IL-5 Cartridge Summary:	Simple Plex assay is for the detection of mouse Interleukin 5 (IL-5) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD09.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Mouse IL-5 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Mouse IL-5 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Mouse
Specificity	Natural and recombinant mouse IL-5.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one IL-5 assay

Inter-Assay Precision (Precision between assays) Replicates of each IL-5 control were tested in multiple assays performed by at least three technicians using two lots of reagents for mouse IL-5

Mouse Cell Culture Supernates, Mouse Serum, Mouse EDTA Plasma, Mouse Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	47	46
Mean (pg/mL)	28.3	1593	24.8	1537
Standard Deviation	1.65	107	2.23	127
CV%	5.8	6.7	9	8.2

Recovery

Recovery for the mouse IL-5 assays was evaluated at three different spiked concentrations of IL-5 protein.

Sample Type	Average % Recovery	Range %
Mouse EDTA Plasma (n=5)	109	98-120
Mouse Heparin Plasma (n=5)	107	98-111
Mouse Serum (n=3)	106	101-115

Linearity

Samples containing and/or spiked with high concentrations of mouse IL-5 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Mouse IL-5 Cartridge

	Simple Plex Mouse IL-5 Assay Standard Curve Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Mouse IL-5 Assay. Assay range is 0.45-4290 pg/mL.

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-5

IL-5 (Interleukin-5) is a secreted cytokine that is expressed as a covalent dimer. It is produced by activated Th2 cells, eosinophils, mast cells, and iNKT cells and promotes eosinophilic and basophilic differentiation and activation. IL-5 signals through a receptor complex containing IL-5 R alpha and the Common beta Chain/CD131 on eosinophils and basophils. Naturally occurring soluble forms of IL-5 R alpha retain the ability to bind IL-5 and limit its bioavailability.

Item	Value
Long Name	Interleukin 5
Entrez Gene IDs	3567 (Human); 16191 (Mouse); 24497 (Rat); 397409 (Porcine); 280825 (Bovine); 403790 (Canine); 100034199 (Equine); 493803 (Feline)
Alternate Names	B-cell differentiation factor I, BCDF mu, BCGFII, EDF, Eo-CSF, Eosinophil differentiation factor, IL-5, IL-5T-cell replacing factor, IL5, TRF, TRFB cell differentiation factor I, interleukin 5 (colony-stimulating factor, eosinophil), interleukin-5

Compatible Instruments

Ella - Automated Immunoassay Platform

Ella Instrument. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Mouse IL-5 Cartridge

Product Datasheets

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COA

Protocols & Manuals

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