Simple Plex Mouse IL-4 Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-MP-009768
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Mouse IL-4 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.
Bronchoalveolar Lavage Fluid, Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 39 | 39 |
| Mean (pg/mL) | 23.7 | 1336 | 22.9 | 1259 |
| Standard Deviation | 0.548 | 55.9 | 1.61 | 81.8 |
| CV% | 2.3 | 4.2 | 7.0 | 6.5 |
Recovery for Simple Plex Mouse IL-4 Cartridge
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Bronchoalveolar Lavage Fluid (n=3) | 97 | 93-101 |
| Cell Culture Media (n=2) | 106 | 105-107 |
| EDTA Plasma (n=4) | 92 | 89-98 |
| Heparin Plasma (n=4) | 78 | 76-81 |
| Serum (n=4) | 80 | 75-84 |
Linearity
Samples containing and/or spiked with high concentrations of mouse IL-4 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Mouse IL-4 Cartridge
Mouse IL-4 Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: IL-4
IL-4 (Interleukin-4) is a cytokine that is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils. It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells. IL-4 plays a dominant role in the development of allergic inflammation and asthma. The type I receptor for IL-4, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
Additional IL-4 Products
Product Documents for Simple Plex Mouse IL-4 Cartridge
Product Specific Notices for Simple Plex Mouse IL-4 Cartridge
For research use only