Simple Plex Mouse IL-1 ra/IL-1F3 Cartridge

Name: Simple Plex Mouse IL-1 ra/IL-1F3 Cartridge
Brand: ProteinSimple
SKU: SPCKB-MP-004019

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-MP-004019

Simple Plex Mouse IL-1ra/IL-1F3 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity:	0.86 pg/mL
Assay Range:	6.43 - 9,806 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Mouse IL-1 ra/IL-1F3 Cartridge Summary:	Simple Plex™ assay is for the detection of mouse Interleukin 1 receptor antagonist (IL-1 ra/IL-1F3) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD09.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Mouse IL-1 ra/IL-1F3 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Mouse IL-1 ra/IL-1F3 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Mouse
Specificity	Natural and recombinant mouse IL-1 ra/IL-1F3.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one IL-1 ra/IL-1F3 assay

Inter-Assay Precision (Precision between assays) Replicates of each IL-1 ra/IL-1F3 control were tested in multiple assays performed by at least three technicians using two lots of reagents for mouse IL-1 ra/IL-1F3

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	91	88
Mean (pg/mL)	110	4905	88.6	3848
Standard Deviation	3.03	153	10.5	412
CV%	2.8	3.1	11.8	10.7

Linearity

Samples containing and/or spiked with high concentrations of mouse IL-1 ra/IL-1F3 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Mouse IL-1 ra/IL-1F3 Cartridge

	Simple Plex Mouse IL-1 ra/IL-1F3 Assay Standard Curve Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Mouse IL-1 ra/IL-1F3 Assay. Assay range is 6.43-9806 pg/mL.

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-1ra/IL-1F3

IL-1ra (IL-1 receptor antagonist) is a widely expressed acute phase protein that serves to dampen inflammation. IL-1ra binds to both IL-1 RI and IL-1 RII. It competitively inhibits IL-1 (alpha or beta) binding to IL-1 RI and does not trigger recruitment of the accessory molecule IL-1 R AcP or activation of IL-1 RI signaling. IL-1ra blocks the proinflammatory actions of IL-1 including Prostaglandin E2 and IL-6 release from endothelial cells, fever generation, thrombocytosis, the production of hepatic acute phase proteins, and neutrophil expansion and infiltration.

Item	Value
Long Name	Interleukin 1 Receptor Antagonist
Entrez Gene IDs	3557 (Human); 16181 (Mouse); 60582 (Rat); 397499 (Porcine); 100034236 (Equine)
Alternate Names	DIRA, ICIL-1ra, IL-1F3, IL-1RN, IL-1ra, IL-1ra3, IL1F3, IL1RN, IL1ra, IRAP, MVCD4, interleukin 1 receptor antagonist

Compatible Instruments

Ella - Automated Immunoassay Platform

Ella Instrument. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Mouse IL-1 ra/IL-1F3 Cartridge

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