Simple Plex Mouse Granzyme B Cartridge New

Name: Simple Plex Mouse Granzyme B Cartridge
Brand: ProteinSimple
SKU: SPCKB-MP-007963

ProteinSimple a Bio-Techne Brand

Catalog # SPCKB-MP-007963

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Key Product Details

Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 pg/mL), Serum (25 pg/mL), EDTA Plasma (25 pg/mL), Heparin Plasma (25 pg/mL)
Sample Types:	Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
Sensitivity:	1.57 pg/mL
Assay Range:	1.57 - 6,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Mouse Granzyme B Cartridge Summary:	Simple Plex assay for the detection of mouse Granzyme B in cell culture supernatant (CCS), serum, and EDTA plasma.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

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Product Specifications for Simple Plex Mouse Granzyme B Cartridge

Reactivity	Mouse
Specificity	Natural and recombinant mouse Granzyme B.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	16	16
Mean (pg/mL)	43.3	2293	44.9	2436
Standard Deviation	1.95	73.6	4.43	198
CV%	4.5	3.2	9.9	8.1

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	117	114-119
Serum (n=4)	114	102-124

Linearity

Samples containing and/or spiked with high concentrations of mouse Granzyme B were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Mouse Granzyme B Cartridge

	Mouse Granzyme B Simple Plex Assay Standard Curve

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Granzyme B

Granzyme family serine proteases are stored in the granules of cytotoxic T lymphocytes and natural killer cells. Granzymes contain one S1 protease domain and are synthesized as preproproteins. Granzymes are released toward pathogen infected or transformed target cells during cellular immune reactions. Target cell entry by granzymes is through perforin channels. Granzymes trigger apoptosis by caspase-dependent and -independent mechanisms. There are five granzymes (A, B, G, H and K) in human and many more in mouse and rat.

Item	Value
Entrez Gene IDs	3002 (Human); 14939 (Mouse)
Alternate Names	C11, CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA-1, CTLA1, CTSGL1, Fragmentin-2, GRB, GZMB, Granzyme B, Granzyme-2, GranzymeB, GrzB, HLP, SECT

Citations for Simple Plex Mouse Granzyme B Cartridge

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