Simple Plex Mouse GDF-15 Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-MP-008561
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Mouse GDF-15 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 60 | 60 |
| Mean (pg/mL) | 8.73 | 416 | 7.83 | 401 |
| Standard Deviation | 0.356 | 26.2 | 0.805 | 29.6 |
| CV% | 4.1 | 6.3 | 10.3 | 7.4 |
Recovery for Simple Plex Mouse GDF-15 Cartridge
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 104 | 104-105 |
Linearity
Samples containing high concentrations of mouse GDF-15 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Mouse GDF-15 Cartridge
Mouse GDF-15 Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: GDF-15
GDF-15 (Growth Differentiation Factor 15) is a dimeric TGF-beta superfamily cytokine that is highly expressed in placenta and brain. It promotes neuronal survival and regulates neuropeptide Y and pro-opiomelanocortin levels to suppress appetite. It is cardioprotective via inhibition of platelet activation, limiting atherosclerosis, promoting recovery following myocardial infarction, and regulating angiogenesis. GDF-15 also inhibits cartilage formation and the production of TNF-alpha production from LPS-stimulated macrophages.
Long Name
Alternate Names
Gene Symbol
Additional GDF-15 Products
Product Documents for Simple Plex Mouse GDF-15 Cartridge
Product Specific Notices for Simple Plex Mouse GDF-15 Cartridge
For research use only