Simple Plex Human VEGFR1/Flt-1 Cartridge Best Seller
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000540
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human VEGFR1/Flt-1 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one VEGFR1 assay.
Inter-Assay Precision (Precision between assays) Replicates of each VEGFR1 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human VEGFR1.
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 19 | 19 |
| Mean (pg/mL) | 27.1 | 1312 | 28.3 | 1336 |
| Standard Deviation | 1.99 | 123 | 1.99 | 119 |
| CV% | 7.3 | 9.4 | 7.0 | 8.9 |
Recovery for Simple Plex Human VEGFR1/Flt-1 Cartridge
Recovery for the human VEGFR1 assays was evaluated at three different spiked concentrations of VEGFR1 protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=3) | 104 | 99-111 |
| Heparin Plasma (n=3) | 81 | 73-88 |
| Serum (n=3) | 97 | 79-111 |
Linearity
Samples containing and/or spiked with high concentra-tions of human VEGF R1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human VEGFR1/Flt-1 Cartridge
Simple Plex Human VEGFR1 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human VEGFR1 Assay. Assay range is 3.05-4650 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: VEGFR1/Flt-1
VEGFR1 (vascular endothelial growth factor receptor 1; Flt-1) binds to VEGF-A, VEGF-B, and PlGF and functions in vasculogenesis, angiogenesis, lymphangiogenesis, and wound healing. It is expressed on endothelial cells, osteoblasts, placental trophoblasts, renal mesangial cells, and some hematopoietic stem cells. It can heterodimerize with VEGFR2 and modulate VEGFR2 mediated endothelial cell proliferation and vessel branching. VEGFR1 engagement on monocyte/macrophage lineage cells enhances their migration, and release of growth factors and cytokines.
Long Name
Alternate Names
Gene Symbol
Additional VEGFR1/Flt-1 Products
Product Documents for Simple Plex Human VEGFR1/Flt-1 Cartridge
Product Specific Notices for Simple Plex Human VEGFR1/Flt-1 Cartridge
For research use only