Simple Plex Human VEGF Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000330
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
0.28 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Assay Range
2.99-4562 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human VEGF Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 8 | 10 |
| Mean (pg/mL) | 34.5 | 1519 | 33.3 | 1440 |
| Standard Deviation | 1.32 | 109 | 2.38 | 89.6 |
| CV% | 3.8 | 7.1 | 7.1 | 6.2 |
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 8 | 10 |
| Mean (pg/mL) | 26.1 | 1126 | 1.33 | 72.3 |
| Standard Deviation | 1.33 | 72.3 | 1.90 | 139 |
| CV% | 5.1 | 6.4 | 6.6 | 10.8 |
Recovery for Simple Plex Human VEGF Cartridge
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cerebrospinal Fluid (n=6) | 114 | 110-119 |
| EDTA Plasma (n=3) | 91 | 82-100 |
| Heparin Plasma (n=3) | 86 | 75-94 |
| Serum (n=3) | 95 | 82-106 |
Linearity
Samples containing and/or spiked with high concentrations of human VEGF-A were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human VEGF Cartridge
Simple Plex Human VEGF-A Assay Standard Curve (Sample Diluent 13)
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human VEGF-A Assay. Assay range is 3.65-5570 pg/mL.
Simple Plex Human VEGF-A Assay Standard Curve (Sample Diluent 06)
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human VEGF-A Assay. Assay range is 2.99-4562 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: VEGF
VEGF (Vascular endothelial growth factor) is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is required for regulating the proliferation, migration, and survival of embryonic endothelial cells and during wound healing and the female reproductive cycle in adults. Pathologically, it is involved in tumor development and tumor vascular leakage. VEGF binds to VEGF R1/Flt-1, VEGF R2/Flk-1/KDR, and Neuropilin-1. Multiple forms of VEGF are generated by alternative splicing and proteolysis.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
Additional VEGF Products
Product Documents for Simple Plex Human VEGF Cartridge
Product Specific Notices for Simple Plex Human VEGF Cartridge
For research use only