Simple Plex Human uPAR Cartridge

Name: Simple Plex Human uPAR Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-007370

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-007370

Product Datasheet / COA

Simple Plex Human uPAR assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sensitivity:	0.2 pg/mL
Assay Range:	1.68 - 16,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Cerebrospinal Fluid)
Simple Plex Human uPAR Cartridge Summary:	Simple Plex assay is for the detection of human Urokinase-type Plasminogen Activator Receptor (uPAR) in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), saliva, urine, and cerebrospinal fluid (CSF) in Sample Diluent SD10.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human uPAR Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human uPAR Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human uPAR.
Cross-reactivity	Cross-reactivity observed with 1 or more available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one uPAR assay

Inter-Assay Precision (Precision between assays) Replicates of each uPAR control were tested in multiple assays performed by at least three technicians using two lots of reagents for human uPAR

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Cerebrospinal Fluid

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	49	50
Mean (pg/mL)	120	6099	110	5748
Standard Deviation	6.8	261	8.51	558
CV%	5.7	4.3	7.7	9.7

Linearity

Samples containing and/or spiked with high concentrations of uPAR were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Human uPAR Cartridge

	Simple Plex Human uPAR Assay Standard Curve Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human uPAR Assay. Assay range is 1.68-16,000 pg/mL.

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: uPAR

The urokinase-type Plasminogen Activator Receptor (uPAR) is a GPI-anchored protein that binds to both the inactive and active forms of uPA. This interaction serves to localize and enhance the proteolytic activity of uPA, leading to increased conversion of Plasminogen to Plasmin. uPA also triggers uPAR-mediated signal transduction, activation of protein tyrosine kinases, gene expression, cell adhesion, and chemotaxis. uPAR can additionally regulate cell adhesion by direct binding to Vitronectin and preventing Integrins from binding to their ligands.

Item	Value
Long Name	Urokinase-type Plasminogen Activator Receptor
Entrez Gene IDs	5329 (Human); 18793 (Mouse); 102139334 (Cynomolgus Monkey)
Alternate Names	CD87, CD87 antigen, Monocyte activation antigen Mo3, PLAUR, U-PAR, UPARurokinase plasminogen activator surface receptor, URKRMO3, plasminogen activator, urokinase receptor, u-plasminogen activator receptor form 2, uPAR

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human uPAR Cartridge

Product Datasheets

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COA

Protocols & Manuals

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