Simple Plex Human TREM-2 Cartridge

Name: Simple Plex Human TREM-2 Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-001847

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-001847

Simple Plex Human TREM-2 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (25 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Cerebrospinal Fluid (10 uL)
Sensitivity:	0.81 pg/mL
Assay Range:	9.96 - 15,200 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)
Simple Plex Human TREM-2 Cartridge Summary:	Simple Plex™ assay is for the detection of human Triggering Receptor Expressed on Myeloid cells 2 (TREM-2) in cell culture supernates (CCS), serum, plasma (EDTA/Heparin), and cerebrospinal fluid (CSF) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human TREM-2 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human TREM-2 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human TREM-2.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	39	39
Mean (pg/mL)	129	6668	124	6144
Standard Deviation	4.81	375	12	619
CV%	3.7	5.6	9.7	10.1

Linearity

Samples containing and/or spiked with high concentrations of human TREM-2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human TREM-2 Cartridge

	Human TREM-2 Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TREM2

The TREM family of receptors regulates the activity of various cell types of the immune system including neutrophils, monocyte/macrophages, microglia, and dendritic cells. The receptors are members of the immunoglobulin superfamily, characterized structurally by a single V-set immunoglobulin (Ig) domain and a short cytoplasmic tail. TREMs associate with the DAP12 signal adapter, an immunoreceptor tyrosine-based activation motif- (ITAM)-containing protein that is phosphorylated and transduces signals when activated.

Item	Value
Long Name	Triggering Receptor Expressed on Myeloid Cells 2
Entrez Gene IDs	54209 (Human); 102133279 (Cynomolgus Monkey)
Alternate Names	PLOSL2, TREM-2, TREM-2triggering receptor expressed on myeloid cells 2a, TREM2, Trem2a, Trem2b, Trem2c, Triggering receptor expressed on monocytes 2, triggering receptor expressed on myeloid cells 2

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human TREM-2 Cartridge

Product Datasheets

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Protocols & Manuals

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