Simple Plex Human TFF3 Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000530
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human TFF3 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one TFF3 assay.
Inter-Assay Precision (Precision between assays) Replicates of each TFF3 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human TFF3.
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 17 | 19 |
| Mean (pg/mL) | 137 | 7796 | 114 | 7221 |
| Standard Deviation | 8.48 | 514 | 14.4 | 647 |
| CV% | 6.2 | 6.6 | 12.6 | 9.0 |
Recovery for Simple Plex Human TFF3 Cartridge
Recovery for the human TFF3 assays was evaluated at three different spiked concentrations of TFF3 protein.
Linearity
Samples containing and/or spiked with high concentrations of human TFF3 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human TFF3 Cartridge
Simple Plex Human TFF3 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human TFF3 Assay. Assay range is 13.1-20,000 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: TFF3
TFF3 (Trefoil Factor 3), also known as ITF and P1.B, is a peptide that plays an important role in epithelial regeneration and wound healing. It can form disulfide-linked dimers or associate into disulfide-linked complexes with the intestinal mucous proteins FCGBP and MUC-2. TFF3 is expressed by epithelial goblet cells in the respiratory tract, biliary and breast ducts, small and large intestine, and cardia of the stomach as well as by chondrocytes during bone development. TFF3 is upregulated in response to gastrointestinal epithelial disruptions and promotes epithelial wound healing. It enhances tumor cell invasion and metastasis by supporting hypoxia-induced VEGF upregulation.
Long Name
Alternate Names
Gene Symbol
Additional TFF3 Products
Product Documents for Simple Plex Human TFF3 Cartridge
Product Specific Notices for Simple Plex Human TFF3 Cartridge
For research use only