Simple Plex Human Pentraxin 3/TSG-14 Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000537
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Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human Pentraxin 3/TSG-14 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Saliva, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 16 | 17 |
| Mean (pg/mL) | 206 | 9568 | 211 | 9837 |
| Standard Deviation | 10.1 | 46 | 11.7 | 552 |
| CV% | 4.9 | 4.8 | 5.5 | 5.6 |
Recovery for Simple Plex Human Pentraxin 3/TSG-14 Cartridge
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=6) | 105 | 103-110 |
| EDTA Plasma (n=4) | 98 | 92-109 |
| Heparin Plasma (n=4) | 102 | 98-109 |
Linearity
Samples containing and/or spiked with high concentrations of human Pentraxin 3 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human Pentraxin 3/TSG-14 Cartridge
Human Pentraxin 3/TSG-14 Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: Pentraxin 3/TSG-14
Pentraxin 3 (PTX3), also known as TNF-stimulated gene 14 (TSG-14), is a secreted glycoprotein belonging to the Pentraxin family of proteins. It is the prototypical long Pentraxin, exhibiting a C-terminal Pentraxin domain characteristic of the family, and a unique N-terminal domain. It is produced by several cell types including endothelial and mononuclear cells. PTX3 acts as a pattern recognition receptor with non-redundant roles in the innate immune response to several microbial agents including the fungal pathogen Aspergillus fumigatus. Overexpression leads to enhanced pro-inflammatory responses. PTX3 is induced in response to LPS and inflammatory cytokines including TNF-alpha and IL-1 beta.
Long Name
Alternate Names
Gene Symbol
Additional Pentraxin 3/TSG-14 Products
Product Documents for Simple Plex Human Pentraxin 3/TSG-14 Cartridge
Product Specific Notices for Simple Plex Human Pentraxin 3/TSG-14 Cartridge
For research use only