Simple Plex Human NF-H Cartridge Best Seller
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000519
This product has been discontinued. Please check out our Simple Plex Discovery NF-H Assay here.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human NF-H Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 10 | 9 |
| Mean (pg/mL) | 43.4 | 2206 | 47.4 | 2881 |
| Standard Deviation | 2.22 | 81.6 | 6.59 | 365 |
| CV% | 5.1 | 3.7 | 13.9 | 12.7 |
Recovery for Simple Plex Human NF-H Cartridge
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=3) | 98 | 93-101 |
| EDTA Plasma (n=4) | 94 | 83-108 |
| Heparin Plasma (n=4) | 102 | 99-107 |
| Serum (n=4) | 102 | 82-114 |
Linearity
Samples containing and/or spiked with high concentrations of human NF-H were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human NF-H Cartridge
Human NF-H Simple Plex Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: NF-H
Neurofilaments are intermediate filaments that are major components of the neuronal cytoskeleton. Neurofilaments are composed of three subunits: the heavy subunit (NF-H), medium subunit (NF-M), and light subunit (NF-L). Neurofilaments are trimers that always contain the 68 kDa NF-L and variably contain 125 kDa NF-M and 200 kDa NF-H. The human neurofilament heavy chain is a phosphorylated cytoskeletal intermediate filament protein that is expressed in neurons. The neurofilament medium subunit Human NF-M is a phosphorylated glycoprotein that belongs to the type IV intermediate filament family. NF-L is a 68 kDa light chain cytoskeletal intermediate filament protein that associates with the 125 kDa NF-M and the 200 kDa NF-H to form neurofilaments.
All three neurofilament subunits share a common structure, with globular head, alpha-helical rod, and carboxy terminal side-arm domains. The subunits assemble to form 10 nm neurofilaments. Accumulation of neurofilaments is seen in several human diseases including amyotrophic lateral sclerosis (ALS), Parkinsons disease, Alzheimers disease, and diabetic neuropathy.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
Additional NF-H Products
Product Documents for Simple Plex Human NF-H Cartridge
Product Specific Notices for Simple Plex Human NF-H Cartridge
For research use only