Simple Plex Human MMP-7 Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000515
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human MMP-7 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one MMP-7 assay.
Inter-Assay Precision (Precision between assays) Replicates of each MMP-7 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human MMP-7.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 21 | 20 |
| Mean (pg/mL) | 38.3 | 2114 | 38.0 | 2088 |
| Standard Deviation | 3.03 | 180 | 4.83 | 202 |
| CV% | 7.9 | 8.5 | 12.7 | 9.7 |
Recovery for Simple Plex Human MMP-7 Cartridge
Recovery for the human MMP-7 assays was evaluated at three different spiked concentrations of MMP-7 protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=2) | 112 | 106-117 |
Linearity
Samples containing and/or spiked with high concentrations of human MMP-7 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human MMP-7 Cartridge
Simple Plex Human MMP-7 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human MMP-7 Assay. Assay range is 14.7-8970 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: MMP-7
Matrix metalloproteinase 7, also known as Matrilysin, is a zinc and calcium dependent endopeptidase that is expressed in epithelial cells of normal and diseased tissues. It can digest a wide range of extracellular matrix proteins including Collagens IV and X, Gelatin, Casein, Laminin, Aggrecan, Entactin, Elastin, and Versican. MMP-7 can activate other proteinases such as Plasminogen, MMP-1, MMP-2, and MMP-9. In addition, MMP-7 regulates the activation or shedding of alpha-Defensin, TNF-alpha, and Fas Ligand.
Long Name
Alternate Names
Gene Symbol
Additional MMP-7 Products
Product Documents for Simple Plex Human MMP-7 Cartridge
Product Specific Notices for Simple Plex Human MMP-7 Cartridge
For research use only