Simple Plex Human LIGHT Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000142
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human LIGHT Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one LIGHT assay.
Inter-Assay Precision (Precision between assays) Replicates of each LIGHT control were tested in multiple assays performed by at least three technicians using two lots of reagents for human LIGHT.
Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 23 | 22 |
| Mean (pg/mL) | 22.2 | 1109 | 20.1 | 1152 |
| Standard Deviation | 0.652 | 59.8 | 1.43 | 64.7 |
| CV% | 2.9 | 5.4 | 7.1 | 5.6 |
Recovery for Simple Plex Human LIGHT Cartridge
Recovery for the human LIGHT assays was evaluated at three different spiked concentrations of LIGHT protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cerebrospinal Fluid (n=6) | 106 | 101-115 |
| EDTA Plasma (n=4) | 97 | 84-103 |
| Heparin Plasma (n=4) | 105 | 100-111 |
| Serum (n=4) | 100 | 86-112 |
Linearity
Samples containing and/or spiked with high concentrations of human LIGHT were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human LIGHT Cartridge
Simple Plex Human LIGHT Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human LIGHT Assay. Assay range is 1.07-4070 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: LIGHT/TNFSF14
LIGHT, also known as TNFSF14, is a homotrimeric glycoprotein in the TNF superfamily. It is expressed on the surface of activated lymphocytes, NK cells, immature dendritic cells, monocytes, and granulocytes. A soluble form can be shed by proteolysis. LIGHT binds and activates HVEM/TNFRSF14 to costimulate Th1 immune responses, enhance CD8+ T cell‑mediated tumor immunity, and regulate allogeneic T cell activation and allograft rejection. In addition, it binds and activates Lymphotoxin beta R/TNFRSF3 to induce apoptosis, cytokine production, mesenteric lymph node organogenesis, and maintenance of lymph node structure and function. It can also bind the soluble human decoy receptor DcR3/TNFRSF6B.
Long Name
Alternate Names
Gene Symbol
Additional LIGHT/TNFSF14 Products
Product Documents for Simple Plex Human LIGHT Cartridge
Product Specific Notices for Simple Plex Human LIGHT Cartridge
For research use only