Simple Plex Human IL-1ra/IL-1F3 Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000323
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human IL-1ra/IL-1F3 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 39 | 35 |
| Mean (pg/mL) | 25.3 | 1310 | 27.2 | 1390 |
| Standard Deviation | 1.47 | 100 | 2.00 | 69.8 |
| CV% | 5.8 | 7.7 | 7.4 | 5.0 |
Recovery for Simple Plex Human IL-1ra/IL-1F3 Cartridge
Recovery was not necessary as IL-1ra shows natural linearity data from a neat sample to a 1:16 dilution.
Linearity
Samples containing and/or spiked with high concentrations of human IL-1ra were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human IL-1ra/IL-1F3 Cartridge
Human IL-1ra Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: IL-1ra/IL-1F3
IL-1ra (IL-1 receptor antagonist) is a widely expressed acute phase protein that serves to dampen inflammation. IL-1ra binds to both IL-1 RI and IL-1 RII. It competitively inhibits IL-1 (alpha or beta) binding to IL-1 RI and does not trigger recruitment of the accessory molecule IL-1 R AcP or activation of IL-1 RI signaling. IL-1ra blocks the proinflammatory actions of IL-1 including Prostaglandin E2 and IL-6 release from endothelial cells, fever generation, thrombocytosis, the production of hepatic acute phase proteins, and neutrophil expansion and infiltration.
Long Name
Alternate Names
Gene Symbol
Additional IL-1ra/IL-1F3 Products
Product Documents for Simple Plex Human IL-1ra/IL-1F3 Cartridge
Product Specific Notices for Simple Plex Human IL-1ra/IL-1F3 Cartridge
For research use only