Simple Plex Human IL-18 BPa Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000502
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human IL-18 BPa Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one IL-18 BPa assay.
Inter-Assay Precision (Precision between assays) Replicates of each IL-18 BPa control were tested in multiple assays performed by at least three technicians using two lots of reagents for human IL-18 BPa.
Bronchoalveolar Lavage Fluid, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 23 | 25 |
| Mean (pg/mL) | 44.9 | 2521 | 43.7 | 2248 |
| Standard Deviation | 2.30 | 232 | 3.22 | 278 |
| CV% | 5.1 | 9.2 | 7.4 | 12.4 |
Recovery for Simple Plex Human IL-18 BPa Cartridge
Recovery was not necessary as human IL-18 BPa shows 5 points of natural linearity.
Linearity
Samples containing and/or spiked with high concentrations of human IL-18 BPa were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human IL-18 BPa Cartridge
Simple Plex Human IL-18 BPa Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human IL-18 BPa Assay. Assay range is 2.84-4336 pg/mL.
Simple Plex Human IL-18 BPa Assay Linearity
Preparation and Storage
Shipping
Stability & Storage
Background: IL-18 BPa
Interleukin 18 binding proteins are secreted glycoproteins which antagonize IL-18 activity by binding IL-18 and blocking its biological activity. Alternative splicing of the IL-18 BP gene produces at least four isoforms that differ in affinity for IL-18. The IL-18 BP isoforms a and c each contain one immunoglobulin (Ig)-like C2-type domain while isoforms b and d lack a complete Ig-like domain.
Long Name
Alternate Names
Gene Symbol
Additional IL-18 BPa Products
Product Documents for Simple Plex Human IL-18 BPa Cartridge
Product Specific Notices for Simple Plex Human IL-18 BPa Cartridge
For research use only