Simple Plex Human HGFR/c-MET Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000325
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human HGFR/c-MET Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one HGF R assay.
Inter-Assay Precision (Precision between assays) Replicates of each HGF R control were tested in multiple assays performed by at least three technicians using two lots of reagents for human HGF R.
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 32 | 33 |
| Mean (pg/mL) | 299 | 13,605 | 272 | 13,483 |
| Standard Deviation | 21.2 | 849 | 23.7 | 1263 |
| CV% | 7.2 | 6.2 | 8.7 | 9.4 |
Recovery for Simple Plex Human HGFR/c-MET Cartridge
Recovery was not necessary as human HGF R shows 5 points of natural linearity.
Linearity
Samples containing and/or spiked with high concentrations of human HGF R were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human HGFR/c-MET Cartridge
Simple Plex Human HGF R Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human HGF R Assay. Assay range is 4.20-100,000 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: HGFR/c-MET
HGFR (Hepatocyte Growth Factor Receptor), also known as Met, is a receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development. In the absence of ligand, HGFR forms noncovalent complexes with a variety of membrane proteins including CD44v6, CD151, EGFR, Fas, Integrin alpha 6 beta 4, Plexins B1, 2, 3, and MSPR/Ron. Ligation of one complex component triggers activation of the other, followed by cooperative signaling effects. Formation of some of these heteromeric complexes is a requirement for epithelial cell morphogenesis and tumor cell invasion. Paracrine induction of epithelial cell scattering and branching tubulogenesis results from the stimulation of HGFR on undifferentiated epithelium by HGF released from neighboring mesenchymal cells.
Long Name
Alternate Names
Gene Symbol
Additional HGFR/c-MET Products
Product Documents for Simple Plex Human HGFR/c-MET Cartridge
Product Specific Notices for Simple Plex Human HGFR/c-MET Cartridge
For research use only