Simple Plex Human Hepcidin Cartridge

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000984

Simple Plex Human Hepcidin assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer. Sample type indicated with PS (Plasma/Serum).

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)

Sensitivity

4.81 pg/mL

Assay Range

9.7-37000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human Hepcidin Cartridge Summary

Simple Plex assay is for the detection of human Hepcidin in serum and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Hepcidin Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Reactivity

Human

Specificity

Natural and recombinant human Hepcidin.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Hepcidin assay.

Inter-Assay Precision (Precision between assays) Replicates of each Hepcidin control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Hepcidin.

EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 48 50
Mean (pg/mL) 209 10,447 218 12,032
Standard Deviation 10.3 789 27.5 1624
CV% 4.9 7.6 12.6 13.5

Recovery for Simple Plex Human Hepcidin Cartridge

Recovery for the human Hepcidin assays was evaluated at three different spiked concentrations of Hepcidin protein.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 109 106-112
Heparin Plasma (n=4) 100 92-113
Serum (n=4) 109 106-117

Linearity

Samples containing and/or spiked with high concentrations of human Hepcidin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Simple Plex Human Hepcidin Assay Linearity

Scientific Data Images for Simple Plex Human Hepcidin Cartridge

Simple Plex Human Hepcidin Assay Standard Curve

Simple Plex Human Hepcidin Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human Hepcidin Assay. Assay range is 9.70-37,000 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Hepcidin

The product encoded by this gene is involved in the maintenance of iron homeostasis, and it is necessary for the regulation of iron storage in macrophages, and for intestinal iron absorption. The preproprotein is post-translationally cleaved into mature peptides of 20, 22 and 25 amino acids, and these active peptides are rich in cysteines, which form intramolecular bonds that stabilize their beta-sheet structures. These peptides exhibit antimicrobial activity. Mutations in this gene cause hemochromatosis type 2B, also known as juvenile hemochromatosis, a disease caused by severe iron overload that results in cardiomyopathy, cirrhosis, and endocrine failure.

Long Name

Hepcidin Antimicrobial Peptide

Alternate Names

HAMP, HEPC, HFE2B, LEAP1, PLTR

Entrez Gene IDs

57817 (Human); 84506 (Mouse); 84604 (Rat)

Gene Symbol

HAMP

Additional Hepcidin Products

Product Documents for Simple Plex Human Hepcidin Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Human Hepcidin Cartridge

For research use only

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