Simple Plex Human GM-CSF Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000493
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human GM-CSF Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 11 | 10 |
| Mean (pg/mL) | 38.5 | 1739 | 38.0 | 1557 |
| Standard Deviation | 1.88 | 40.7 | 2.11 | 111 |
| CV% | 4.9 | 2.3 | 5.6 | 7.1 |
Recovery for Simple Plex Human GM-CSF Cartridge
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=4) | 98 | 91-111 |
| Heparin Plasma (n=4) | 96 | 93-99 |
| Serum (n=4) | 94 | 82-107 |
Linearity
Samples containing and/or spiked with high concentrations of human GM-CSF were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human GM-CSF Cartridge
Human GM-CSF Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: GM-CSF
GM-CSF (Granulocyte-Macrophage Colony Stimulating Factor) induces the development of monocytes, neutrophils, eosinophils, and myeloid and dermal dendritic cells. It also acts as a neutrophil and dendritic cell chemoattractant. GM-CSF promotes Th1 and Th17 cell mediated autoimmune inflammation as well as the inflammatory activation of dendritic cells, microglia, alveolar macrophages, and eosinophils. In addition, it cooperates with G-CSF in promoting tumor cell proliferation and invasion. GM-CSF signals through a receptor complex composed of GM-CSF R alpha and the Common beta Chain (beta c) with Syndecan-2 as a potential co-receptor. The beta c subunit is shared by the receptor complexes for IL-3 and IL-5.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
Additional GM-CSF Products
Product Documents for Simple Plex Human GM-CSF Cartridge
Product Specific Notices for Simple Plex Human GM-CSF Cartridge
For research use only