Simple Plex Human GFAP Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-HF-000970
Simple Plex Human GFAP has been discontinued and is no longer available. We have replaced it with the Human GFAP 2nd gen assay SPCKB-PS-009134.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human GFAP Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents
Cerebrospinal Fluid
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 40 | 40 |
| Mean (pg/mL) | 603 | 27073 | 709 | 30982 |
| Standard Deviation | 18.7 | 1217 | 78.1 | 3591 |
| CV% | 3.1 | 4.5 | 11.0 | 11.6 |
Recovery for Simple Plex Human GFAP Cartridge
Recovery was not necessary as GFAP shows natural linearity data from a neat sample to a 1:16 dilution.
Linearity
Samples containing and/or spiked with high concentrations of human GFAP were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human GFAP Cartridge
Human GFAP Simple Plex Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: GFAP
Glial Fibrillary Acidic Protein (GFAP) is a type III intermediate filament protein. GFAP is the predominant component of astrocyte intermediate filaments in the central nervous system and is often used as an astrocytic marker. It has also been detected in the glial cells of the enteric nervous system and some Schwann cells in the peripheral nervous system.
Long Name
Alternate Names
Gene Symbol
Additional GFAP Products
Product Documents for Simple Plex Human GFAP Cartridge
Product Specific Notices for Simple Plex Human GFAP Cartridge
For research use only