Simple Plex Human FGF-21 Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000401
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human FGF-21 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one FGF-21 assay.
Inter-Assay Precision (Precision between assays) Replicates of each FGF-21 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human FGF-21.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 23 | 22 |
| Mean (pg/mL) | 34.9 | 1705 | 33.6 | 1654 |
| Standard Deviation | 2.86 | 131 | 2.63 | 60.1 |
| CV% | 8.2 | 7.7 | 7.8 | 3.6 |
Recovery for Simple Plex Human FGF-21 Cartridge
Recovery for the human FGF-21 assays was evaluated at three different spiked concentrations of FGF-21 protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=4) | 111 | 106-119 |
| EDTA Plasma (n=4) | 107 | 99-113 |
| Heparin Plasma (n=4) | 108 | 97-122 |
| Serum (n=4) | 97 | 85-103 |
Linearity
Samples containing and/or spiked with high concentrations of human FGF-21 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human FGF-21 Cartridge
Simple Plex Human FGF-21 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human FGF-21 Assay. Assay range is 8.93-5450 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: FGF-21
FGF-21 (Fibroblast Growth Factor 21) is a member of an FGF subfamily that also includes FGF-19 and FGF-23. FGF-19 subfamily members lack a heparin binding domain, enabling them to freely circulate as endocrine factors and diffuse within tissues. FGF-21 signals through Klotho beta in complex with FGF receptors. It regulates cellular metabolism including glucose uptake in adipocytes and cellular sensitivity to insulin. It is basally expressed in the pancreas, thymus, liver, and adipose tissue and is upregulated in liver, muscle, and fat in obesity and diabetes. FGF-21 also plays a role in cell survival and proliferation, mesenchymal stem cell differentiation, circadian rhythm, and controlling reproductive capacity.
Long Name
Alternate Names
Gene Symbol
Additional FGF-21 Products
Product Documents for Simple Plex Human FGF-21 Cartridge
Product Specific Notices for Simple Plex Human FGF-21 Cartridge
For research use only