Simple Plex Human Ferritin Cartridge

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-001044

Simple Plex Human Ferritin assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer. Sample type indicated with PS (Plasma/Serum).

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (25 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Cerebrospinal Fluid (10 µL)

Sensitivity

2.9 pg/mL

Assay Range

16.6-63240 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)

Simple Plex Human Ferritin Cartridge Summary

Simple Plex assay is for the detection of human Ferritin cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and cerebrospinal fluid (CSF) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Ferritin Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Reactivity

Human

Specificity

Natural and recombinant human Ferritin.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Ferritin assay.

Inter-Assay Precision (Precision between assays) Replicates of each Ferritin control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Ferritin.

Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 32 32
Mean (µL) 355 20,037 362 20,915
Standard Deviation 12.3 1650 28.8 2318
CV% 3.5 8.2 8.0 11.1

Recovery for Simple Plex Human Ferritin Cartridge

Recovery was not necessary as human Ferritin shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of human Ferritin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Simple Plex Human Ferritin Assay Linearity

Scientific Data Images for Simple Plex Human Ferritin Cartridge

Simple Plex Human Ferritin Assay Standard Curve

Simple Plex Human Ferritin Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human Ferritin Assay. Assay range is 16.6-636,240 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Ferritin

Alternate Names

apoferritin, Cell proliferation-inducing gene 15 protein, Ferritin H subunit, ferritin heavy chain, ferritin, heavy polypeptide 1, FHC, FTHEC 1.16.3.1, FTHL6MGC104426, PIG15, placenta immunoregulatory factor, PLIF, proliferation-inducing protein 15

Entrez Gene IDs

2495 (Human)

Gene Symbol

FTH1

Additional Ferritin Products

Product Documents for Simple Plex Human Ferritin Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Human Ferritin Cartridge

For research use only

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