Simple Plex Human FABP2/I-FABP Cartridge

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000361

Simple Plex Human FABP2/I-FABP assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer. Sample type indicated with PS (Plasma/Serum).

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

5.66 pg/mL

Assay Range

31.1-118560 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human FABP2/I-FABP Cartridge Summary

Simple Plex assay is for the detection of human Fatty Acid Binding Protein 2 (FABP2) in serum and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all FABP2/I-FABP Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Reactivity

Human

Specificity

Natural and recombinant human FABP2.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one FABP2 assay.

Inter-Assay Precision (Precision between assays) Replicates of each FABP2 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human FABP2.

EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 47 47
Mean (pg/mL) 792 37,335 777 36,690
Standard Deviation 43.0 842 67.0 2714
CV% 5.4 2.3 8.6 7.4

Recovery for Simple Plex Human FABP2/I-FABP Cartridge

Recovery for the human FABP2 assays was evaluated at three different spiked concentrations of FABP2 protein.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 102 95-109
Heparin Plasma (n=3) 102 100-106
Serum (n=4) 104 86-113

Linearity

Samples containing and/or spiked with high concentrations of human FABP2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Simple Plex Human FABP2 Assay Linearity

Scientific Data Images for Simple Plex Human FABP2/I-FABP Cartridge

Simple Plex Human FABP2 Assay Standard Curve

Simple Plex Human FABP2 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human FABP2 Assay. Assay range is 31.1 - 118,560 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: FABP2/I-FABP

Fatty acid binding proteins are small cytoplasmic lipid binding proteins that are expressed in a tissue specific manner. FABPs bind free fatty acids, cholesterol, and retinoids, and are involved in intracellular lipid transport. Circulating FABP levels are used as indicators of tissue damage. Some FABP polymorphisms have been associated with disorders of lipid metabolism and the development of atherosclerosis.

FABP2, also known as intestinal fatty acid binding protein (I-FABP or FABPI) and gut FABP (gFABP), is a member of the cytosolic fatty acid binding protein family. FABP2 mediates the absorption and intracellular transport of dietary long-chain fatty acids. Genetic variations of FABP2 are implicated in obesity and Type II diabetes. Human FABP2 shares 78%, 82%, and 86% amino acid sequence identity with mouse, rat, and canine FABP2, respectively.

Long Name

Fatty Acid-Binding Protein 2/Intestinal FABP

Alternate Names

I-FABP, IFABP, Intestinal FABP

Entrez Gene IDs

2169 (Human); 25598 (Rat)

Gene Symbol

FABP2

Additional FABP2/I-FABP Products

Product Documents for Simple Plex Human FABP2/I-FABP Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Human FABP2/I-FABP Cartridge

For research use only

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