Simple Plex Human ENPP-2/Autotaxin Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-001780
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human ENPP-2/Autotaxin Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one ENPP-2 assay.
Inter-Assay Precision (Precision between assays) Replicates of each ENPP-2 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human ENPP-2.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 32 | 32 |
| Mean (pg/mL) | 723 | 41,109 | 766 | 36,307 |
| Standard Deviation | 33.1 | 2677 | 77.8 | 3520 |
| CV% | 4.6 | 6.5 | 10.2 | 9.7 |
Recovery for Simple Plex Human ENPP-2/Autotaxin Cartridge
Recovery was not necessary as human ENPP-2 shows 5 points of natural linearity.
Linearity
Samples containing and/or spiked with high concentrations of human ENPP-2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human ENPP-2/Autotaxin Cartridge
Simple Plex Human ENPP-2 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human ENPP-2 Assay. Assay range is 31.8-121,440 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: ENPP-2/Autotaxin
ENPP-2, also known as Autotaxin, belongs to the ectonucleotide pyrophosphatase/phosphodiesterase (NPP) family. Some NPPs hydrolyze phosphates from nucleotides and their derivatives. ENPP-2 shares 40 - 50% identity to ENPP1 & 3, all of which contain a N-terminal intracellular domain, a single transmembrane domain and a large extracellular domain that includes a catalytic domain, two somatomedin-B-like domains, and a C-terminal nuclease-like domain. Unlike ENPP-1 and ENPP-3, ENPP-2 has weak activity against nucleotides, but exhibits a lysophospholipase D activity which allows the formation of lysophosphatidic acid (LPA) and choline from lysophosphatidylcholine. The hydrolysis of nucleotides and lysophospholipids by ENPP-2 is mediated by a single catalytic site. Evidence shows LPA and sphingosine 1-phosphate to be specific inhibitors of ENPP-2. ENPP-2 was originally found to stimulate tumor cell motility and has since been found to enhance tumor invasion and metastasis and to be up-regulated in several types of carcinomas including breast and lung.
Long Name
Alternate Names
Gene Symbol
Additional ENPP-2/Autotaxin Products
Product Documents for Simple Plex Human ENPP-2/Autotaxin Cartridge
Product Specific Notices for Simple Plex Human ENPP-2/Autotaxin Cartridge
For research use only