Simple Plex Human Cyr61/CCN1 Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-002307
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human Cyr61/CCN1 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Cyr61 assay.
Inter-Assay Precision (Precision between assays) Replicates of each Cyr61 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Cyr61.
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 46 | 43 |
| Mean (pg/mL) | 158 | 6549 | 159 | 6772 |
| Standard Deviation | 9.33 | 499 | 15.8 | 618 |
| CV% | 5.9 | 7.6 | 9.9 | 9.1 |
Recovery for Simple Plex Human Cyr61/CCN1 Cartridge
Recovery for the human Cyr61 assays was evaluated at three different spiked concentrations of Cyr61 protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=4) | 100 | 91-111 |
| Heparin Plasma (n=4) | 96 | 83-103 |
| Serum (n=4) | 95 | 85-105 |
Linearity
Samples containing and/or spiked with high concentrations of human Cyr61 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human Cyr61/CCN1 Cartridge
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human Cyr61 Assay. Assay range is 4.86-18,532 pg/mL.
Preparation and Storage
Shipping
Stability & Storage
Background: Cyr61/CCN1
Cyr61, also known as IGFBP-10 and CCN1, is a 50 kDa matricellular glycoprotein that regulates the growth and adhesion of vascular endothelial cells, fibroblasts, and monocytes. Cyr61 induces VEGF upregulation, angiogenesis, and tumorigenesis through interactions with integrins alpha V beta 3, alpha V beta 5, alpha M beta 2, and alpha 6 beta 1. Cyr61 is cleaved by plasmin within its VWF domain which generates an N-terminal fragment that is not associated with the matrix but retains the ability to induce endothelial cell migration.
Long Name
Alternate Names
Gene Symbol
Additional Cyr61/CCN1 Products
Product Documents for Simple Plex Human Cyr61/CCN1 Cartridge
Product Specific Notices for Simple Plex Human Cyr61/CCN1 Cartridge
For research use only