Simple Plex Human CD40/TNFRSF5 Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000196
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human CD40/TNFRSF5 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one CD40 assay.
Inter-Assay Precision (Precision between assays) Replicates of each CD40 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human CD40.
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 17 | 16 |
| Mean (pg/mL) | 226 | 11,174 | 230 | 11,840 |
| Standard Deviation | 6.90 | 526 | 9.92 | 973 |
| CV% | 3.0 | 4.7 | 4.3 | 8.2 |
Recovery for Simple Plex Human CD40/TNFRSF5 Cartridge
Recovery was not necessary as human CD40 shows 5 points of natural linearity.
Linearity
Samples containing and/or spiked with high concentrations of human CD40 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human CD40/TNFRSF5 Cartridge
Simple Plex Human CD40 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human CD40 Assay. Assay range is 4.28-16,324 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: CD40/TNFRSF5
CD40, also known as TNFRSF5, is a transmembrane TNF superfamily receptor that is expressed on the surface of B cells, dendritic cells, macrophages, monocytes and platelets, as well as endothelial and epithelial cells. Interaction of CD40 with CD40 Ligand leads to the aggregation of CD40 molecules resulting in the initiation of bidirectional intracellular signaling in both CD40 and CD40 Ligand expressing cells. This interaction promotes B cell activation and T cell-dependent humoral responses. CD40 serves multiple functions in both hematopoietic and epithelial cancers and is a target for tumor immunotherapy. Dysregulation of CD40/CD40 Ligand expression and interactions contributes to disease severity in AIDS, atherosclerosis, atherothrombosis, and restenosis.
Alternate Names
Gene Symbol
Additional CD40/TNFRSF5 Products
Product Documents for Simple Plex Human CD40/TNFRSF5 Cartridge
Product Specific Notices for Simple Plex Human CD40/TNFRSF5 Cartridge
For research use only