Simple Plex Human CD40 Ligand/TNFSF5 Cartridge

Name: Simple Plex Human CD40 Ligand/TNFSF5 Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-011041

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-011041

Simple Plex Human CD40 Ligand/TNFSF5 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD06, and wash buffer.

Customize Your Simple Plex Cartridge

Create your custom Simple Plex cartridge for up to 8 targets. Choose from more than 250 targets across multiple species.

Build My Cartridge

Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Platelet-poor EDTA Plasma (25 uL), Heparin Plasma (25 uL), Platelet-poor Heparin Plasma (25 uL)
Sensitivity:	1.47 pg/mL
Assay Range:	3.45 - 5,262 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Platelet-poor EDTA Plasma, Heparin Plasma, Platelet-poor Heparin Plasma)
Simple Plex Human CD40 Ligand/TNFSF5 Cartridge Summary:	Simple Plex assay is for the detection of human CD40 Ligand in cell culture supernatant (CCS), serum, and platelet-poor plasma (EDTA/Heparin) in Sample Diluent SD06.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human CD40 Ligand/TNFSF5 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human CD40 Ligand/TNFSF5 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human CD40 Ligand.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one CD40 Ligand assay

Inter-Assay Precision (Precision between assays) Replicates of each CD40 Ligand control were tested in multiple assays performed by at least three technicians using two lots of reagents for human CD40 Ligand

Cell Culture Supernates, Serum, EDTA Plasma, Platelet-poor EDTA Plasma, Heparin Plasma, Platelet-poor Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	42	42
Mean (pg/mL)	48.6	2441	42.2	2031
Standard Deviation	1.99	80.5	4.21	198
CV%	4.1	3.3	10	9.8

Recovery

Recovery for the human CD40 Ligand assays was evaluated at three different spiked concentrations of CD40 protein.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	101	95-107
EDTA Plasma (n=4)	87	81-88
Heparin Plasma (n=4)	90	85-95
Platelet-poor EDTA Plasma (n=4)	96	93-101
Platelet-poor Heparin Plasma (n=4)	90	87-96

Linearity

Samples containing and/or spiked with high concentrations of human CD40 Ligand were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Human CD40 Ligand/TNFSF5 Cartridge

	Simple Plex Human CD40 Ligand Assay Standard Curve Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human CD40 Ligand Assay. Assay range is 3.45-5262 pg/mL.

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD40 Ligand/TNFSF5

CD40 Ligand, also known as TNFSF5, CD154, TRAP, and gp39, is a trimeric protein that is expressed in transmembrane and soluble forms. It is expressed by many immune cell types as well as by endothelial and smooth muscle cells of the vasculature. It binds to CD40 on antigen presenting cells, platelets, and endothelial and epithelial cells, leading to enhanced B cell activation and T cell dependent humoral immune responses. CD40 Ligand dysregulation contributes to immune pathology in AIDS, atherosclerosis, atherothrombosis, and restenosis.

Entrez Gene IDs

959 (Human); 21947 (Mouse); 84349 (Rat)

Alternate Names

CD154, CD154 antigen, CD40 Ligand, CD40 antigen ligand, CD40-L, CD40L, CD40LG, CD40LIGM, HIGM1, T-B cell-activating molecule, T-BAM, T-cell antigen Gp39, TNF-related activation protein, TNFSF5, TNFSF5IMD3, TRAP, TRAPtumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome), Tumor necrosis factor ligand superfamily member 5, gp39, hCD40L, tumor necrosis factor (ligand) superfamily member 5

Compatible Instruments

Ella - Automated Immunoassay Platform

Ella Instrument. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

Learn More

Product Documents for Simple Plex Human CD40 Ligand/TNFSF5 Cartridge

Product Datasheets

Download Product Insert

COA

Protocols & Manuals

Download Protocol