Simple Plex Human CD27/TNFRSF7 Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000639
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human CD27/TNFRSF7 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one CD27 assay.
Inter-Assay Precision (Precision between assays) Replicates of each CD27 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human CD27.
Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 15 | 15 |
| Mean (pg/mL) | 346 | 14787 | 310 | 14320 |
| Standard Deviation | 15.2 | 1440 | 20.7 | 960 |
| CV% | 4.4 | 9.7 | 6.7 | 6.7 |
Recovery for Simple Plex Human CD27/TNFRSF7 Cartridge
Recovery for the human CD27 assays was evaluated at three different spiked concentrations of CD27 protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=7) | 102 | 94-117 |
Linearity
Samples containing and/or spiked with high concentrations of human CD27 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human CD27/TNFRSF7 Cartridge
Simple Plex Human CD27 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human CD27 Assay. Assay range is 30.7-46,900 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: CD27/TNFRSF7
Human CD27 is a lymphocyte-specific member of the TNF receptor superfamily. CD27 is expressed on a subset of human thymocytes and on the majority of mature T cells. CD27 expression is induced by antigen-receptor activation in B cells.
Alternate Names
Gene Symbol
Additional CD27/TNFRSF7 Products
Product Documents for Simple Plex Human CD27/TNFRSF7 Cartridge
Product Specific Notices for Simple Plex Human CD27/TNFRSF7 Cartridge
For research use only