Simple Plex Human CCL20/MIP-3 alpha Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-001170
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human CCL20/MIP-3 alpha Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one CCL20/MIP-3 alpha assay.
Inter-Assay Precision (Precision between assays) Replicates of each CCL20/MIP-3 alpha control were tested in multiple assays performed by at least three technicians using two lots of reagents for human CCL20/MIP-3 alpha.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 31 | 28 |
| Mean (pg/mL) | 21.1 | 992 | 19.9 | 918 |
| Standard Deviation | 1.30 | 55.3 | 1.90 | 67.6 |
| CV% | 6.2 | 5.6 | 9.7 | 7.2 |
Recovery for Simple Plex Human CCL20/MIP-3 alpha Cartridge
Recovery for the human CCL20/MIP-3 alpha assays was evaluated at three different spiked concentrations of CCL20/MIP-3 alpha protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 103 | 100-106 |
| EDTA Plasma (n=4) | 102 | 97-107 |
| Heparin Plasma (n=4) | 100 | 92-111 |
| Serum (n=4) | 101 | 100-103 |
Linearity
Samples containing and/or spiked with high concentrations of human MIP-3 alpha were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human CCL20/MIP-3 alpha Cartridge
Simple Plex Human CCL20/MIP-3 alpha Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human CCL20/MIP-3 alpha Assay. Assay range is 0.32-3100 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: CCL20/MIP-3 alpha
CCL20, also known as MIP-3 alpha, is a chemokine that plays an important role in dendritic cell trafficking and recruitment and activation of T cells. It is upregulated in monocytes, T cells, endothelial cells, epithelial cells, and fibroblasts following inflammatory stimulation. CCL20 signals through CCR6 which is expressed on memory T cells, B cells and bone marrow-derived dendritic cells.
Alternate Names
Gene Symbol
Additional CCL20/MIP-3 alpha Products
Product Documents for Simple Plex Human CCL20/MIP-3 alpha Cartridge
Product Specific Notices for Simple Plex Human CCL20/MIP-3 alpha Cartridge
For research use only