Simple Plex Human CCL17/TARC Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000194
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human CCL17/TARC Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one TARC assay.
Inter-Assay Precision (Precision between assays) Replicates of each TARC control were tested in multiple assays performed by at least three technicians using two lots of reagents for human TARC.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 14 | 13 |
| Mean (pg/mL) | 6.33 | 417 | 6.84 | 393 |
| Standard Deviation | 0.298 | 38.9 | 0.541 | 41.8 |
| CV% | 4.7 | 9.3 | 7.9 | 10.6 |
Recovery for Simple Plex Human CCL17/TARC Cartridge
Recovery for the human TARC assays was evaluated at three different spiked concentrations of TARC protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 109 | 107-111 |
| EDTA Plasma (n=4) | 99 | 84-112 |
| Heparin Plasma (n=4) | 97 | 79-110 |
| Serum (n=4) | 115 | 98-122 |
Linearity
Samples containing and/or spiked with high concentrations of human TARC were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human CCL17/TARC Cartridge
Simple Plex Human TARC Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human TARC Assay. Assay range is 0.56-850 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: CCL17/TARC
CCL17/TARC is a chemokine that interacts with CCR4 and CCR8 to induce the chemoattraction of activated Th2 cells, basophils, and NK cells. It is produced by thymic dendritic cells, lymph node dendritic cells, monocytes, CD4+ T cells, keratinocytes, fibroblasts, bronchial epithelial cells, and Reed-Sternberg cells. CCL17 promotes Th2 cell recruitment to sites of inflammation and induces platelet aggregation and degranulation.
Additional CCL17/TARC Products
Product Documents for Simple Plex Human CCL17/TARC Cartridge
Product Specific Notices for Simple Plex Human CCL17/TARC Cartridge
For research use only