Simple Plex Human Carbonic Anhydrase IX/CA9 Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-001194
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human Carbonic Anhydrase IX/CA9 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one CA9 assay.
Inter-Assay Precision (Precision between assays) Replicates of each CA9 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human BMP-9.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 33 | 32 |
| Mean (pg/mL) | 100 | 4692 | 104 | 4671 |
| Standard Deviation | 4.36 | 357 | 7.92 | 417 |
| CV% | 4.4 | 7.6 | 7.6 | 8.9 |
Recovery for Simple Plex Human Carbonic Anhydrase IX/CA9 Cartridge
Recovery for the human CA9 assays was evaluated at three different spiked concentrations of CA9 protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=4) | 103 | 94-118 |
| Heparin Plasma (n=4) | 100 | 93-105 |
| Serum (n=4) | 110 | 106-117 |
Linearity
Samples containing and/or spiked with high concentrations of human CA9 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human Carbonic Anhydrase IX/CA9 Cartridge
Simple Plex Human CA9 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human CA9 Assay. Assay range is 4.47-17,060 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: Carbonic Anhydrase IX/CA9
Carbonic anhydrases catalyze the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. Human members of the CAs are secreted (CA6), membrane-bound (CA4, 9 to 12 and 14), cytosolic (CA1 to 3, 7, 8 and 13) or mitochondrial (CA5A and 5B). Some CAs may serve as markers for tumors and hypoxia. A subset of CAs lack CA activity due to point mutations but retain esterase function.
Additional Carbonic Anhydrase IX/CA9 Products
Product Documents for Simple Plex Human Carbonic Anhydrase IX/CA9 Cartridge
Product Specific Notices for Simple Plex Human Carbonic Anhydrase IX/CA9 Cartridge
For research use only