Simple Plex Human CA125/MUC16 Cartridge

Name: Simple Plex Human CA125/MUC16 Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000475

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000475

Simple Plex Human CA125/MUC16 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity:	158 mU/mL
Assay Range:	309.28 - 471,920 mU/mL (Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human CA125/MUC16 Cartridge Summary:	Simple Plex assay is for the detection of human Cancer Antigen 125 (CA125/MUC16) in serum and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human CA125/MUC16 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human CA125/MUC16 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human CA125.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one CA125 assay

Inter-Assay Precision (Precision between assays) Replicates of each CA125 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human CA125

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	21	18
Mean (mU/mL)	2954	141869	2885	130993
Standard Deviation	55.9	3239	152	8524
CV%	1.9	2.3	5.3	6.5

Recovery

Recovery for the human CA125 assays was evaluated at three different spiked concentrations of CA125 protein.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	101	97-106
Heparin Plasma (n=4)	93	90-98
Serum (n=4)	95	88-99

Linearity

Samples containing and/or spiked with high concentrations of CA125 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Human CA125/MUC16 Cartridge

	Simple Plex Human CA125 Assay Standard Curve Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human CA125 Assay. Assay range is 309-471,920 pg/mL.

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CA125/MUC16

The CA125 antigen, also known as MUC16, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy. Expression of isoforms, proteolytic cleavage, and heavy N- and O- linked glycosylation produce forms of human CA125 that can vary from 200-5000 kDa in size. It inhibits NK cell activity, which is thought to contribute to immune evasion in peritoneal cancer and pregnancy.

Item	Value
Long Name	Ovarian Cancer Antigen CA125
Entrez Gene IDs	94025 (Human)
Alternate Names	CA-125, CA125, CA125 ovarian cancer antigen, CA125MUC-16, FLJ14303, MUC16, Ovarian cancer-related tumor marker CA125, Ovarian carcinoma antigen CA125, mucin 16, cell surface associated, mucin-16

Compatible Instruments

Ella - Automated Immunoassay Platform

Ella Instrument. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human CA125/MUC16 Cartridge

Product Datasheets

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Protocols & Manuals

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