Simple Plex Human Angiopoietin-2 Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000244
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human Angiopoietin-2 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 7 | 8 |
| Mean (pg/mL) | 163 | 7021 | 155 | 7324 |
| Standard Deviation | 8.68 | 454 | 13.9 | 298 |
| CV% | 5.3 | 6.5 | 9.0 | 4.1 |
Recovery for Simple Plex Human Angiopoietin-2 Cartridge
Recovery was not necessary as Angiopoietin-2 shows 5 points of natural linearity.
Linearity
Samples containing and/or spiked with high concentrations of human Angiopoietin-2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human Angiopoietin-2 Cartridge
Human Angiopoietin-2 Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: Angiopoietin-2
Angiopoietin-2 (Ang-2; also ANGPT2) is a secreted glycoprotein that regulates vascular remodeling by promoting endothelial cell survival, proliferation, and migration and destabilizing the interaction between EC and perivascular cells. Ang-2 is required for postnatal vascular remodeling, and it cooperates with Ang-1 during lymphatic vessel development. It mediates the upregulation of ICAM-1 and VCAM-1 on EC, which facilitates the adhesion of leukocytes during inflammation. Its direct interaction with Integrins promotes tumor cell invasion. Ang-2 is widely expressed during development, but it is restricted postnatally to highly angiogenic tissues such as the placenta, ovaries, and uterus. It associates into dimers, trimers, and larger multimers and binds the receptor Tie-2, with its function as an agonist or antagonist depending on its state of multimerization.
Alternate Names
Entrez Gene IDs
Gene Symbol
Additional Angiopoietin-2 Products
Product Documents for Simple Plex Human Angiopoietin-2 Cartridge
Product Specific Notices for Simple Plex Human Angiopoietin-2 Cartridge
For research use only