Simple Plex Discovery Mouse IL-10 Cartridge New
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-MP-017052
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Discovery Mouse IL-10 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one IL-10 assay.
Inter-Assay Precision (Precision between assays) Replicates of each IL-10 control were tested in multiple assays performed by at least three technicians using two lots of reagents for mouse IL-10.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 34 | 37 |
| Mean (pg/mL) | 50.8 | 2807 | 53.6 | 2765 |
| Standard Deviation | 1.79 | 178 | 5.32 | 278 |
| CV% | 3.5 | 6.3 | 9.9 | 10.1 |
Recovery for Simple Plex Discovery Mouse IL-10 Cartridge
Recovery for the mouse IL-10 assays was evaluated at three different spiked concentrations of IL-10 protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 106 | 102-109 |
| EDTA Plasma (n=2) | 105 | 105-106 |
| Heparin Plasma (n=3) | 73 | 71-75 |
| Serum (n=2) | 80 | 75-86 |
Linearity
Samples containing and/or spiked with high concentrations of IL-10 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Discovery Mouse IL-10 Cartridge
Simple Plex Discovery Mouse IL-10 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Discovery Mouse IL-10 Assay. Assay range is 6.01-9174 pg/mL.
Simple Plex Discovery Mouse IL-10 Assay Endogenous Levels
Functional LOD and functional LLOQ are calculated as the LOD and LLOQ of the assay multiplied by the minimum required dilution (MRD) for the sample type.Preparation and Storage
Shipping
Stability & Storage
Background: IL-10
IL-10 (Interleukin-10) functions as an anti-inflammatory cytokine by inhibiting the expansion and activation of Th1 cells and Th17 cells and by promoting the development of M2 macrophages and regulatory T cells (Treg). Within a tumor microrenvironment, however, IL-10 can inhibit the expansion of both Treg and myeloid-derived suppressor cells (MDSC). IL-10 exerts protective effects including limiting tissue damage in arthritic inflammation and promoting muscle regeneration after injury, but it also contributes to the persistence of viral infections. IL-10 signals through a receptor complex composed of IL-10 R alpha and IL-10 R beta. IL-10 R beta additionally associates with IL-20 R alpha, IL-22 R alpha 1, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
Additional IL-10 Products
Product Documents for Simple Plex Discovery Mouse IL-10 Cartridge
Product Specific Notices for Simple Plex Discovery Mouse IL-10 Cartridge
For research use only