Simple Plex Discovery Human vWF-A2 Cartridge New
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-016135
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Discovery Human vWF-A2 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one vWF-A2 assay.
Inter-Assay Precision (Precision between assays) Replicates of each vWF-A2 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human vWF-A2.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 43 | 44 |
| Mean (pg/mL) | 164 | 7310 | 154 | 7067 |
| Standard Deviation | 6.16 | 442 | 18.1 | 669 |
| CV% | 3.8 | 6.0 | 11.8 | 9.5 |
Recovery for Simple Plex Discovery Human vWF-A2 Cartridge
Recovery for the human vWF-A2 assays was evaluated at three different spiked concentrations of vWF-A2 protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 111 | 111-112 |
| EDTA Plasma (n=2) | 93 | 91-94 |
| Heparin Plasma (n=2) | 102 | 98-105 |
| Serum (n=2) | 90 | 89-91 |
Linearity
Samples containing and/or spiked with high concentrations of vWF-A2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Discovery Human vWF-A2 Cartridge
Simple Plex Discovery Human vWF-A2 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Discovery Human vWF-A2 Assay. Assay range is 13.1-20,000 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: vWF-A2
Human vWF is a serum glycoprotein that mediates platelet adhesion to injured vascular endothelium. vWF function is regulated by ADAMTS13 mediated proteolysis within the A2 domain. Mutations of this protease cause thrombotic thrombocytopenic purpura.
Long Name
Alternate Names
Gene Symbol
Additional vWF-A2 Products
Product Documents for Simple Plex Discovery Human vWF-A2 Cartridge
Product Specific Notices for Simple Plex Discovery Human vWF-A2 Cartridge
For research use only