Simple Plex Discovery Human TSLP Assay

Name: Simple Plex Discovery Human TSLP Assay
Brand: ProteinSimple
SKU: SPCKB-PS-011826

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-011826

Simple Plex Human TSLP assay kit for use on Ella instrument. Contains a cartridge, Sample Diluent SD13, and Wash Buffer. Sample type indicated with PS (Plasma/Serum).

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity:	0.09 pg/mL
Assay Range:	0.21 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Discovery Human TSLP Assay Summary:	Simple Plex Discovery assay is for the detection of human Thymic Stromal Lymphopoietin (TSLP) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Discovery Human TSLP Assay

Technical Data
Citations

Product Specifications for Simple Plex Discovery Human TSLP Assay

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human TSLP.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	25	27
Mean (pg/mL)	11.6	546	12.2	583
Standard Deviation	0.367	17.9	0.885	30.3
CV%	3.2	3.3	7.3	5.2

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	110	107-113
EDTA Plasma (n=2)	86	84-87
Heparin Plasma (n=2)	99	96-102
Serum (n=2)	92	92-92

Linearity

Samples containing and/or spiked with high concentrations of recombinant human TSLP were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Discovery Human TSLP Assay

	Simple Plex Discovery TSLP Ella Assay Standard Curve

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TSLP

Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. The activities of mouse TSLP overlap with, but are distinct from, those of mouse IL-7. Both mouse TSLP and IL-7 can co-stimulate growth of thymocytes and mature T cells, and support B lymphopoiesis in long-term cultures of fetal liver cells and bone-marrow cells. Whereas mouse IL-7 facilitates the development of B220⁺/IgM^-pre-B cells, mouse TSLP promotes the development B220⁺/IgM⁺ B cells. Human TSLP was reported to preferentially stimulate myeloid cells, inducing the release of T cell-attracting chemokines from monocytes and enhancing the maturation of CD11c⁺ dendritic cells. Human TSLP cDNA encodes a 159 amino acid (aa) residue precursor protein with a 28 aa signal sequence. Within the mature region, six of the seven cysteine residues present in the mouse TSLP involved in intramolecular disulfide bond formation are conserved in the human TSLP. Human TSLP shares approximately 43% aa sequence identity with mouse TSLP. By Northern blot analysis, human TSLP expression has been detected in many tissues with the highest expressions in heart, liver, testis and prostate. TSLP signals through a heterodimeric receptor complex that consists of IL-7 R alpha and the TSLP R, a member of the hemopoietin receptor family most closely related to Rgamma_c.

TSLP R, also named Delta and CRLM-2 (Cytokine Receptor-like Module-2), was originally cloned as a novel type 1 cytokine receptor with similarity to the common gamma chain (gamma c). It was subsequently identified as a subunit of the cellular receptor for the IL-7-like cytokine TSLP and termed TSLP R. TSLP R shows 24% sequence identity with the mouse gc receptor. TSLP R expression is constitutive in immune and hematopoietic cells, but is up-regulated in Th2 cells.

Item	Value
Long Name	Thymic Stromal Lymphopoietin
Entrez Gene IDs	85480 (Human); 53603 (Mouse); 102119254 (Cynomolgus Monkey)
Alternate Names	TSLP, thymic stromal lymphopoietin

Product Documents for Simple Plex Discovery Human TSLP Assay

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Simple Plex Discovery Human TSLP Assay

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-011826

Customize Your Simple Plex Cartridge

Key Product Details

Product Specifications for Simple Plex Discovery Human TSLP Assay

Precision

Recovery

Linearity

Scientific Data Examples for Simple Plex Discovery Human TSLP Assay

Preparation and Storage

Background: TSLP

Product Documents for Simple Plex Discovery Human TSLP Assay

Certificate of Analysis

CONTACT INFORMATION

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Simple Plex Discovery Human TSLP Assay

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-011826

Customize Your Simple Plex Cartridge

Key Product Details

Product Specifications for Simple Plex Discovery Human TSLP Assay

Precision

Recovery

Linearity

Scientific Data Examples for Simple Plex Discovery Human TSLP Assay

Preparation and Storage

Background: TSLP

Product Documents for Simple Plex Discovery Human TSLP Assay

Product Datasheets

Protocols & Manuals

COA

Certificate of Analysis

SDS