Simple Plex Discovery Human Syndecan-1 Assay

Name: Simple Plex Discovery Human Syndecan-1 Assay
Brand: ProteinSimple
SKU: SPCKB-PS-014654

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-014654

Simple Plex Human Syndecan-1 assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and Wash Buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Urine (10 uL)
Sensitivity:	0.61 pg/mL
Assay Range:	5.24 - 20,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Simple Plex Discovery Human Syndecan-1 Assay Summary:	Simple Plex Discovery assay is for the detection of human Syndecan-1 in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and urine in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Discovery Human Syndecan-1 Assay

Technical Data
Citations

Product Specifications for Simple Plex Discovery Human Syndecan-1 Assay

Assay Type	utomated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human Syndecan-1.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Syndecan-1 assay

Inter-Assay Precision (Precision between assays) Replicates of each Syndecan-1 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Syndecan-1

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	36	36
Mean (pg/mL)	158	8446	155	7808
Standard Deviation	6.08	526	11.7	717
CV%	3.8	6.2	7.5	9.2

Recovery

Recovery for the human Syndecan-1 assays was evaluated at three different spiked concentrations of Syndecan-1 protein.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	103	102-103

Linearity

Samples containing and/or spiked with high concentrations of Syndecan-1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Discovery Human Syndecan-1 Assay

	Simple Plex Discovery Human Syndecan-1 Assay Standard Curve Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Discovery Human Syndecan-1 Assay. Assay range is 5.24-20,000 pg/mL.

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Syndecan-1/CD138

Syndecans are type 1 transmembrane proteoglycans that constitute the major physiological form of heparan sulfate (HS) on the cell surface. The four vertebrate syndecans, Syndecan-1 through -4, have similar short cytoplasmic domains and extracellular portions that diverge, except for HS attachment sites. Structurally diverse side chains add considerably to the size of the core proteins and serve as binding sites for growth factors, cytokines, and extracellular matrix proteins. Syndecans are present as homodimers or multimers, and are often expressed in developmental and cell type-specific patterns. They have many biological roles including regulating cell growth, differentiation, and adhesion.

Item	Value
Entrez Gene IDs	6382 (Human); 20969 (Mouse); 100519770 (Porcine); 100338470 (Rabbit)
Alternate Names	CD138, CD138 antigen, SDC, SDC1, SYND1heparan sulfate proteoglycan fibroblast growth factor receptor, Syndecan-1, Syndecan1, syndecan, syndecan 1, syndecan proteoglycan 1

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.