Simple Plex Discovery Human Pro-Collagen I alpha 1

Name: Simple Plex Discovery Human Pro-Collagen I alpha 1
Brand: ProteinSimple
SKU: SPCKB-PS-015353

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-015353

Simple Plex Human Pro-Collagen Ia1 assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and Wash Buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity:	3 pg/mL
Assay Range:	26.4 - 40,348 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Discovery Human Pro-Collagen I alpha 1 Summary:	Simple Plex Discovery assay is for the detection of human Pro-Collagen I alpha 1 (Pro-Collagen I alpha 1) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Discovery Human Pro-Collagen I alpha 1

Technical Data
Citations

Product Specifications for Simple Plex Discovery Human Pro-Collagen I alpha 1

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human Pro-Collagen I alpha 1.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Pro-Collagen I alpha 1 assay

Inter-Assay Precision (Precision between assays) Replicates of each Pro-Collagen I alpha 1 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Pro-Collagen I alpha 1

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	18	18
Mean (pg/mL)	339	16608	346	16281
Standard Deviation	15.1	403	40	1256
CV%	4.4	2.4	11.6	7.7

Recovery

Recovery for the human Pro-Collagen I alpha 1 assays was evaluated at three different spiked concentrations of Pro-Collagen I alpha 1 protein.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	96	95-98

Linearity

Samples containing and/or spiked with high concentrations of Pro-Collagen I alpha 1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Discovery Human Pro-Collagen I alpha 1

	Simple Plex Discovery Human Pro-Collagen I alpha 1 Assay Standard Curve Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Discovery Human Pro-Collagen I alpha 1 Assay. Assay range is 26.4-40.348 pg/mL.

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Pro-Collagen I alpha 1

Type I collagen is the most abundant structural protein of connective tissues such as skin, bone and tendon. It is synthesized as a procollagen molecule which is characterized by a 300 nm triple helical domain flanked by globular N- and C-terminal propeptides. The triple helical domain contains Gly-Xaa-Yaa triplets where Xaa and Yaa are frequently proline and hydroxyproline, respectively. The non-helical propeptides are removed by procollagen N- and C-proteinase activities so that the mature triple helices can self-assemble into collagen fibrils that provide tensile strength to tissues. Type I collagen is a heterotrimer that consists of two alpha 1(I) chains and one alpha 2(I) chain, although homotrimers consisting of three identical alpha 1(I) chains have also been described. This recombinant mini pro-alpha 1(I) collagen consists of a shortened alpha 1(I) chain with following domain structure from N- to C-terminus: N-propeptide, N-telopeptide, the 33 most N-terminal Gly-Xaa-Yaa repeats, the 33 most C-terminal Gly-Xaa-Yaa repeats, C-telopeptide and C-propeptide. The preparation contains a mixture of the full-length molecule, pN collagen I (alpha 1) and the C-terminal propeptide. This truncated pro-alpha 1(I) collagen is a substrate for procollagen N-proteinase and procollagen C-proteinase.

Item	Value
Long Name	Pro-Collagen I alpha 1
Alternate Names	Pro-Collagen I alpha 1

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Discovery Human Pro-Collagen I alpha 1

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