Simple Plex Discovery Human PD-L2/B7-DC Assay
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-012638
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Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Discovery Human PD-L2/B7-DC Assay Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 22 | 22 |
| Mean (pg/mL) | 22.1 | 941 | 22.8 | 1044 |
| Standard Deviation | 0.572 | 26.8 | 2.63 | 72.1 |
| CV% | 2.6 | 2.9 | 11.5 | 6.9 |
Recovery for Simple Plex Discovery Human PD-L2/B7-DC Assay
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 99 | 98-100 |
Linearity
Samples containing and/or spiked with high concentrations of human PD-L2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Discovery Human PD-L2/B7-DC Assay
Simple Plex Discovery Human PD-L2/B7-DC Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: PD-L2/B7-DC
PD-L2 (Programmed Death Ligand 2), also known as B7-DC and butyrophilin-like protein, is a transmembrane protein expressed on dendritic cells, subsets of activated CD4+ and CD8+ T cells, and memory B cells that differentiate into plasma cells. At inflammatory sites, PD-L2 is upregulated on synoviocytes, infiltrating macrophages, dendritic cells, and airway epithelial cells. PD-L2, along with B7-H1/PD-L1, binds to T cell PD-1 where it promotes IFN-gamma production and CD40 Ligand upregulation while inhibiting IL-4 production. In addition, PD-L2 binds to RGM-B on macrophages and alveolar epithelial cells, supporting respiratory immune tolerance. In asthma, PD-L2 suppresses IL-5 and IL-13 production, promotes IL-12 production by dendritic cells, and supports allergen-induced airway hyper-responsiveness and mucus production.
Long Name
Alternate Names
Gene Symbol
Additional PD-L2/B7-DC Products
Product Documents for Simple Plex Discovery Human PD-L2/B7-DC Assay
Product Specific Notices for Simple Plex Discovery Human PD-L2/B7-DC Assay
For research use only