Simple Plex Discovery Human OX40/TNFRSF4 Assay

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-012637

Simple Plex Human OX40 assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and Wash Buffer. Sample type indicated with PS (Plasma/Serum).

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

0.28 pg/mL

Assay Range

1.05-4000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Discovery Human OX40/TNFRSF4 Assay Summary

Simple Plex Discovery assay is for the detection of human OX40 in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13. 

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all OX40 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Reactivity

Human

Specificity

Natural and recombinant human OX40.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 27 27
Mean (pg/mL) 35.9 1519 33.3 1465
Standard Deviation 1.05 43.0 3.06 119
CV% 2.9 2.8 9.2 8.1

Recovery for Simple Plex Discovery Human OX40/TNFRSF4 Assay

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 106 105-108

Linearity

Samples containing and/or spiked with high concentrations of human OX40 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Discovery Human OX40/TNFRSF4 Ella Assay

Scientific Data Images for Simple Plex Discovery Human OX40/TNFRSF4 Assay

Simple Plex Discovery Human OX40/TNFRSF4 Ella Assay

Simple Plex Discovery Human OX40/TNFRSF4 Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: OX40

OX40 (CD134; TNFRSF4) is a T cell costimulatory molecule of the TNF receptor superfamily that coordinates with other costimulators (CD28, CD40, CD30, CD27 and 4-1BB) to manage the activation of the immune response. OX40 is upregulated on antigen activated CD4+ and CD8+ T cells with costimulation by CD40-CD40 Ligand and CD28-B7. OX40 interactions with OX40 Ligand on antigen presenting cells enhances T cell survival, proliferation, and cytokine production. It also inhibits the conversion of effector T cells into regulatory T cells (Tregs) and can promote the maintenance of and recall response in memory T cells. OX40 is constitutively expressed on Tregs where it promotes Treg proliferation and immunosuppressive activity. OX40-OX40 Ligand signaling is involved in allergic airway inflammation, graft-versus-host disease, and autoimmune disease.

Alternate Names

ACT-135, CD134, Ly-70, TNFRSF4, Txgp1, TXGP1L

Entrez Gene IDs

7293 (Human); 22163 (Mouse); 25572 (Rat); 102145978 (Cynomolgus Monkey)

Gene Symbol

TNFRSF4

Additional OX40 Products

Product Documents for Simple Plex Discovery Human OX40/TNFRSF4 Assay

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Certificate of Analysis

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Product Specific Notices for Simple Plex Discovery Human OX40/TNFRSF4 Assay

For research use only

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