Simple Plex Discovery Human MBL Assay
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-012016
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Discovery Human MBL Assay Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 23 | 24 |
| Mean (pg/mL) | 87.3 | 4484 | 89.4 | 4324 |
| Standard Deviation | 1.60 | 159 | 4.37 | 206 |
| CV% | 2.6 | 3.5 | 4.9 | 4.8 |
Recovery for Simple Plex Discovery Human MBL Assay
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 90 | 88-93 |
Linearity
Samples containing and/or spiked with high concentrations of recombinant human MBL were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Discovery Human MBL Assay
Simple Plex Discovery Human MBL Ella Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: MBL
Mannose/mannan-binding lectin (MBL) is a secreted glycoprotein in the collectin family of pattern-recognition molecules. It contains a cysteine-rich region, a collagen-like segment, and a C-type lectin domain that binds to neutral bacterial carbohydrates. The collagen-like region mediates MBL association into disulfide-stabilized trimers which further associate into complexes containing three or four copies of the basic trimer. MBL is secreted by hepatocytes and opsonizes bacteria through interactions with microbial carbohydrates. MBL multimers can associate with the serine proteases MASP-1, -2, and -3 and promote their cleavage of Complement Component C3. Proteolytic cleavage of C3 triggers activation of the complement system and formation of the membrane attack complex, leading to destruction of opsonized bacteria. Opsonized apoptotic material is cleared by the scavenger receptor CD91.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
Additional MBL Products
Product Documents for Simple Plex Discovery Human MBL Assay
Product Specific Notices for Simple Plex Discovery Human MBL Assay
For research use only