Simple Plex AAV3 Cartridge

Name: Simple Plex AAV3 Cartridge
Brand: ProteinSimple
SKU: SPCKB-OT-012247

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-OT-012247

Product Datasheet / COA

Simple Plex AAV3 assay kit contains cartridge, Sample Diluent SD19, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Bioprocess Samples (25 uL)
Sensitivity:	0.37 10^6 capsids/mL
Assay Range:	3.54 - 5,400 10^6 capsids/mL (Bioprocess Samples)
Simple Plex AAV3 Cartridge Summary:	Simple Plex assay is for the detection of Adeno-Associated Virus type 3 (AAV3) intact capsids in Bioprocess samples in Sample Diluent SD19.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex AAV3 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex AAV3 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Viral
Specificity	This assay recognizes intact AAV3 capsids.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents

Bioprocess Samples

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	37	54
Mean (10<sup>6</sup> capsids/mL)	6.4	161	7.48	171
Standard Deviation	0.3	4.6	0.51	11.6
CV%	4.5	2.9	6.8	6.7

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Bioprocess Samples (n=6)	97	94-99

Linearity

Samples containing and/or spiked with high concentrations of AAV3 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex AAV3 Cartridge

	Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex AAV3 Assay. Assay range is 3.54-5,400 10⁶ capsids/mL.

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: AAV3

Item	Value
Long Name	Adeno-associated Virus 3
Alternate Names	AAV3

Compatible Instruments

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Product Documents for Simple Plex AAV3 Cartridge

Product Datasheets

Download Product Insert

COA

Protocols & Manuals

Download Protocol