Simple Plex AAV3 Cartridge

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-OT-012247

Simple Plex AAV3 assay kit contains cartridge, Sample Diluent SD19, and wash buffer.
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ST01B-OT-012247
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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Bioprocess Samples (25 µL)

Sensitivity

0.37 106 capsids/mL

Assay Range

3.54-5400 106 capsids/mL (Bioprocess Samples)

Simple Plex AAV3 Cartridge Summary

Simple Plex assay is for the detection of Adeno-Associated Virus type 3 (AAV3) intact capsids in Bioprocess samples in Sample Diluent SD19. 

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all AAV3 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Reactivity

Viral

Specificity

This assay recognizes intact AAV3 capsids. 

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents.

Bioprocess Samples

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 37 54
Mean (10<sup>6</sup> capsids/mL) 6.40 161 7.48 171
Standard Deviation 0.300 4.60 0.51 11.6
CV% 4.5 2.9 6.8 6.7

Recovery for Simple Plex AAV3 Cartridge

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Bioprocess Samples (n=6) 97 94-99

Linearity

Samples containing and/or spiked with high concentrations of AAV3 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex AAV3 Assay Linearity

Scientific Data Images for Simple Plex AAV3 Cartridge

Simple Plex AAV3 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex AAV3 Assay. Assay range is 3.54-5,400 106 capsids/mL.

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: AAV3

Long Name

Adeno-associated Virus 3

Additional AAV3 Products

Product Documents for Simple Plex AAV3 Cartridge

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Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex AAV3 Cartridge

For research use only

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