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Non-Human Primate Flt-3 Ligand Cartridge

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-NP-011356

Simple Plex Non-Human Primate Flt-3 Ligand assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer. Sample type indicated by NP (Plasma/Serum).

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)

Sensitivity

0.07 pg/mL

Assay Range

0.16-3868 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Non-Human Primate Flt-3 Ligand Cartridge Summary

Simple Plex assay for the detection of non-human primate Flt-3 Ligand/FLT3L in serum and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Reactivity

Non-human Primate

Specificity

Natural and recombinant Flt-3 Ligand. 

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.

EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 44 42
Mean (pg/mL) 22.9 1163 22.3 1069
Standard Deviation 0.6 60.6 2.16 95.1
CV% 2.7 5.2 9.7 8.9

Recovery for Non-Human Primate Flt-3 Ligand Cartridge

Recovery was not necessary as Flt-3 Ligand shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of Flt-3 Ligand were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Images for Non-Human Primate Flt-3 Ligand Cartridge

Non-Human Primate Flt-3 Ligand Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Flt-3 Ligand/FLT3L

Flt-3 Ligand, also known as FLT3L, is a cytokine that promotes the differentiation or expansion of multiple hematopoietic cell lineages including monocytes, immature dendritic cells, and early B cell lineage cells. It synergizes with other cytokines to promote the myeloid differentiation and mobilization of hematopoietic stem cells, to induce NK cell development, and to induce terminal B cell maturation. Flt-3 Ligand/FLT3L is expressed as a dimer by T cells and bone marrow and thymic fibroblasts. Both transmembrane and soluble forms of Flt-3 Ligand/FLT3L signal through the tyrosine kinase receptor Flt-3/Flk-2.

Long Name

fms-like Tyrosine Kinase 3 Ligand

Alternate Names

FLG3L, Flt3L, FLT3LG

Entrez Gene IDs

2323 (Human); 14256 (Mouse); 102125470 (Cynomolgus Monkey); 493796 (Feline)

Gene Symbol

FLT3LG

Additional Flt-3 Ligand/FLT3L Products

Product Documents for Non-Human Primate Flt-3 Ligand Cartridge

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Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Non-Human Primate Flt-3 Ligand Cartridge

For research use only

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