Non-Human Primate CD163 Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-NP-011437
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Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Non-Human Primate CD163 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 28 | 30 |
| Mean (pg/mL) | 191 | 9427 | 87 | 4299 |
| Standard Deviation | 8.3 | 641 | 10.2 | 400 |
| CV% | 4.3 | 6.8 | 11.7 | 9.3 |
Recovery for Non-Human Primate CD163 Cartridge
Recovery was not necessary as CD163 shows 5 points of natural linearity.
Linearity
Samples containing and/or spiked with high concentrations of CD163 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Non-Human Primate CD163 Cartridge
Non-Human Primate CD163 Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: CD163
CD163, also known as M130 and p155, is a transmembrane scavenger receptor that is expressed on monocytes and macrophages and is inducible by immunosuppressant glucocorticoids and IL-10. A soluble form is shed from the cell surface by TACE or neutrophil elastase mediated cleavage in response to oxidative stress, Prostaglandin F2a stimulation, or the activation of Fc gamma receptors, TLR1, 2, 5, or 6. CD163 mediates monocyte binding to bacteria, leading to the release of inflammatory cytokines. It is essential for the circulatory clearance of hemoglobin-haptoglobin (Hb-Hp) complexes as well as free hemoglobin. It can also mediate monocyte-erythroblast adhesion and promote erythroblast expansion. CD163 binds and internalizes the cytokine TWEAK, and the ratio of soluble CD163 to TWEAK in the plasma is elevated during atherosclerosis.
Alternate Names
Gene Symbol
Additional CD163 Products
Product Documents for Non-Human Primate CD163 Cartridge
Product Specific Notices for Non-Human Primate CD163 Cartridge
For research use only