Goat anti-Rat IgA Heavy Chain Secondary Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-60686
![Western Blot: Goat anti-Rat IgA Heavy Chain Secondary Antibody [NBP2-60686]](https://resources.bio-techne.com/images/products/Goat-anti-Rat-IgA-Secondary-Antibody-Unconjugated-Pre-adsorbed-Western-Blot-NBP2-60686-img0001.jpg "Western Blot: Goat anti-Rat IgA Heavy Chain Secondary Antibody [NBP2-60686]")
Discontinued Product
NBP2-60686 has been discontinued.
View all IgA Heavy Chain products.
Key Product Details
Species Reactivity
Rat
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Rat IgA alpha heavy chain
Specificity
No reaction was observed against Rat IgM or Rat IgG. Specificity was confirmed by ELISA at less than 1% cross reactivity against other Rat heavy or light chain isotypes.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgA and Rat Serum
Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Scientific Data Images for Goat anti-Rat IgA Heavy Chain Secondary Antibody
Western Blot: Goat anti-Rat IgA Heavy Chain Secondary Antibody [NBP2-60686]
Western Blot: Goat anti-Rat IgA Heavy Chain Secondary Antibody [NBP2-60686] - Lane 1: Mouse IgA Kappa. Lane 2: None. Load: 100 ng per lane. Primary antibody: Rat IgA (Alpha chain) antibody biotin conjugated at 1:1,000 for 60 min at RT. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:40,000 for 30 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Rat IgA. Other band(s): None. Image using the Biotin form of this antibody.![Western Blot: Goat anti-Rat IgA Heavy Chain Secondary Antibody [NBP2-60686]](https://resources.bio-techne.com/images/products/Goat-anti-Rat-IgA-Secondary-Antibody-Unconjugated-Pre-adsorbed-Western-Blot-NBP2-60686-img0002.jpg "Western Blot: Goat anti-Rat IgA Heavy Chain Secondary Antibody [NBP2-60686]")
Western Blot: Goat anti-Rat IgA Heavy Chain Secondary Antibody [NBP2-60686]
Western Blot: Goat anti-Rat IgA Heavy Chain Secondary Antibody [NBP2-60686] - Lane 1: Mouse IgA Kappa. Lane 2: None. Load: 100 ng per lane. Primary antibody: None. Secondary antibody: Texas Red goat secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Rat IgA. Other band(s): Rat IgA light chain. Image using the Texas Red form of this antibody.Applications for Goat anti-Rat IgA Heavy Chain Secondary Antibody
Application
Recommended Usage
ELISA
1:10000 - 1:50000
Immunohistochemistry
1:500 - 1:3000
Western Blot
1:1000-1:5000
Application Notes
This product is suitable for Elisa, Western Blots and Immunohistochemistry. Antibody should be optimized by the end user for specific conditions of reactivity.
Formulation, Preparation, and Storage
Purification
Multi-step
Formulation
0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IgA Heavy Chain
Additional IgA Heavy Chain Products
Product Documents for Goat anti-Rat IgA Heavy Chain Secondary Antibody
Product Specific Notices for Goat anti-Rat IgA Heavy Chain Secondary Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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