Recombinant Yeast Ubiquitin Activating Enzyme (UBE1), CF

Name: Recombinant Yeast Ubiquitin Activating Enzyme (UBE1), CF
Brand: R&D Systems
SKU: E-301

R&D Systems, part of Bio-Techne | Catalog # E-301

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Product Datasheet / COA / SDS

Discontinued Product

E-301 has been discontinued. View all Ubiquitin-activating Enzyme/UBE1 products.

Key Product Details

Source:	Sf 9 (baculovirus)
Accession #:	P22515
Applications:	Enzyme Activity

View Full Technical Details for Recombinant Yeast Ubiquitin Activating Enzyme (UBE1), CF

Technical Data
Citations / Reviews

Product Specifications for Recombinant Yeast Ubiquitin Activating Enzyme (UBE1), CF

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-301

Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ubiquitin-activating Enzyme/UBE1

Ubiquitin-activating Enzyme (UBE1), also known as Ubiquitin-like Modifier Activating Enzyme 1 (UBA1), is a 1058 amino acid (aa) canonical member of the Ubiquitin-activating (E1) enzyme family of proteins with a predicted molecular weight of 118 kDa. It is ubiquitously expressed and highly conserved; mouse and rat UBE1 share 95% and 96% aa sequence identity with the human UBE1 protein, respectively. UBE1 is found in the cytoplasm and nucleus, and contains a conserved active-site cysteine residue and ATP-binding site common to E1 enzymes. UBE1 is responsible for the first step in Ubiquitin-protein isopeptide bond formation. Ubiquitin is activated by UBE1 and thereafter linked to the side chain of a cysteine residue in UBE1, Cys632 in humans, yielding a Ubiquitin-UBE1 conjugate via a thioester bond. The activated Ubiquitin is then transferred to a lysine residue on the target protein via the Ubiquitin-conjugating - Ubiquitin ligase enzyme cascade. UBE1 is required for cell cycle progression and has been linked to cellular responses to DNA damage such as nucleotide excision repair. Mutations in UBE1 are associated with X-linked lethal infantile spinal muscular atrophy. UBE1 is a critical component for the initiation of in vitro ubiquitin conjugation reactions.

Item	Value
References	Ciechanover A. et al. (1982) J. Biol. Chem. 257:2537-2542 Ghaboosi N. and Deshaies R. J. (2007) Mol. Biol. Cell. 181:1953-1963 Haas A. L. and Rose I. A. (1982) J. Biol. Chem. 257:1032910337 Haas A. L. et al. (1982) J. Biol. Chem. 257:2543-2548 Handley P. M. et al. (1991) Proc. Natl. Acad. Sci. 88:258-262 Hoefer M and Cook J. C. (1991) FEBS. Lett. 289:54-59 Jonnalagadda S. et al. (1988) J. Biol. Chem. 263:5016-5019 MacGrath J. P. et al. (1991) EMBO J. 10:227-236 Pickart C. M. et al. (1994) J. Biol. Chem. 269:7115-7123 Salvat C. et al. (2000) Eur. J. Biochem. 267:3712-3722 Stephen A. G. et al. (1996) J. Biol. Chem. 271:15608-15614 Wilkinson K. D. et al. (1990) Biochem. 29:7373-7380
Entrez Gene IDs	7317 (Human); 22201 (Mouse); 314432 (Rat); 100009225 (Rabbit); 853670 (Yeast)
Alternate Names	A1S9T, A1S9T and BN75 temperature sensitivity complementing, A1ST, AMCX1, GXP1, MGC4781, POC20, POC20 centriolar protein homolog, Protein A1S9, SMAX2, UBA1, UBA1, ubiquitin-activating enzyme E1 homolog A, UBA1A, UBE1, UBE1A1S9, UBE1X, Ubiquitin-activating Enzyme, Ubiquitin-activating enzyme E1, Ubiquitinactivating Enzyme, ubiquitin-activating enzyme E1 (A1S9T and BN75 temperature sensitivitycomplementing), ubiquitin-like modifier activating enzyme 1, ubiquitin-like modifier-activating enzyme 1

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Recombinant Yeast Ubiquitin Activating Enzyme (UBE1), CF

R&D Systems, part of Bio-Techne | Catalog # E-301

Key Product Details

Product Specifications for Recombinant Yeast Ubiquitin Activating Enzyme (UBE1), CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Ubiquitin-activating Enzyme/UBE1

Customer Reviews for Recombinant Yeast Ubiquitin Activating Enzyme (UBE1), CF

Product Documents for Recombinant Yeast Ubiquitin Activating Enzyme (UBE1), CF

Product Datasheets

COA

Certificate of Analysis

SDS

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