Recombinant SARS-CoV-2 Spike (Mink Cluster-5) His Protein CF

Name: Recombinant SARS-CoV-2 Spike (Mink Cluster-5) His Protein CF
Brand: R&D Systems
SKU: 10736-CV

Catalog # 10736-CV | R&D Systems, Inc. a Bio-Techne Brand

Setting the standard in quality research reagents

Key Product Details

Source:	HEK293
Accession #:	YP_009724390.1

View Full Technical Details for Recombinant SARS-CoV-2 Spike (Mink Cluster-5) His Protein CF

Technical Data
Citations / Reviews

Product Specifications for Recombinant SARS-CoV-2 Spike (Mink Cluster-5) His Protein CF

Source	Human embryonic kidney cell, HEK293-derived sars-cov-2 Spike protein Val16-Lys1211 (His69-Val70 del & Tyr453Phe, Asp614Gly, Ile692Val)(Arg682Ser, Arg685Ser, Lys986Pro, Val987Pro), with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Val 16
Predicted Molecular Mass	134 kDa
SDS-PAGE	134 kDa

Scientific Data Examples for Recombinant SARS-CoV-2 Spike (Mink Cluster-5) His Protein CF

	Recombinant SARS-CoV-2 Spike (Mink Cluster-5) His-tag Protein Bioactivity Recombinant SARS-CoV-2 Spike (Mink Cluster-5) His-tag (Catalog # 10736-CV) binds Recombinant Human ACE-2 His-tag (933-ZN) in a functional ELISA.
	Recombinant SARS-CoV-2 Spike (Mink Cluster-5) His-tag Protein SDS-PAGE. 2 μg/lane of Recombinant SARS-CoV-2 Spike (Mink Cluster-5) His-tag (Catalog # 10736-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 154-170 kDa.
	Binding of ACE-2 to Mink Cluster-5 variant Spike protein by surface plasmon resonance (SPR). Recombinant SARS-CoV-2 Mink Cluster-5 variant Spike protein His-tag was immobilized on a Biacore Sensor Chip CM5, and binding to recombinant human ACE-2 (933-ZN) was measured at a concentration range between 0.18 nM and 47.2 nM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=0.3411 nM. (Biacore T200).

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10736-CV

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Spike

SARS-CoV-2, which causes the global pandemic coronavirus disease 2019 (Covid-19), belongs to a family of viruses known as coronaviruses that are commonly comprised of four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein (N) (1). SARS-CoV-2 Spike Protein (S Protein) is a glycoprotein that mediates membrane fusion and viral entry. The S protein is homotrimeric, with each ~180-kDa monomer consisting of two subunits, S1 and S2 (2). In SARS-CoV-2, as with most coronaviruses, proteolytic cleavage of the S protein into the S1 and S2 subunits is required for activation. The S1 subunit is focused on attachment of the protein to the host receptor while the S2 subunit is involved with cell fusion (3-5). A SARS-CoV-2 variant carrying the S protein amino acid (aa) change D614G has become the most prevalent form in the global pandemic and has been associated with greater infectivity and higher viral load (6, 7). The S protein of SARS-CoV-2 shares 75% and 29% aa sequence identity with S protein of SARS-CoV-1 and MERS, respectively. The S Protein of the SARS-CoV-2 virus, like the SARS-CoV-1 counterpart, binds Angiotensin-Converting Enzyme 2 (ACE2), but with much higher affinity and faster binding kinetics through the receptor binding domain (RBD) located in the C-terminal region of S1 (8). It has been demonstrated that the S Protein can invade host cells through the CD147/EMMPRIN receptor and mediate membrane fusion (9, 10). Cluster 5, also known as DFVI-spike is a variant of the virus strain that was first discovered in Denmark and infected more than 200 mink farms by the end of November 2020 (11). Evidence shows virus has been transmitted from mink farms to human in a zoonotic manner (12). WHO claims that this mutant has moderately decreased sensitivity to the neutralization antibody.

Item	Value
References	Wu, F. et al. (2020) Nature 579:265. Tortorici, M.A. and D. Veesler (2019) Adv. Virus Res. 105:93. Bosch, B.J. et al. (2003) J. Virol. 77:8801. Belouzard, S. et al. (2009) Proc. Natl. Acad. Sci. 106:5871. Millet, J.K. and G.R. Whittaker (2015) Virus Res. 202:120. Korber, B. et al. (2020) Cell 182:812. Zhang, L. et al. (2020) bioRxiv https://www.biorxiv.org/content/10.1101/2020.06.12.148726v1. Ortega, J.T. et al. (2020) EXCLI J. 19:410. Wang, X. et al. (2020) https://doi.org/10.1038/s41423-020-0424-9. Wang, K. et al. (2020) bioRxiv https://www.biorxiv.org/content/10.1101/2020.03.14.988345v1. Larsen, HD. et al. (2021) Eurosurveilance 26:2100009. Munnink, B.B.O. et al. (2021) Science 371:172.
Long Name	Spike Protein
Entrez Gene IDs	918758 (HCoV-229E); 2943499 (HCoV-NL63); 39105218 (HCoV-OC43); 37616432 (MERS-CoV); 1489668 (SARS-CoV); 43740568 (SARS-CoV-2)
Alternate Names	2019-nCoV S Protein, 2019-nCoV Spike, COVID-19 Spike, E2, Human coronavirus spike glycoprotein, Peplomer protein, S Protein, S glycoprotein, SARS-COV-2 S protein, SARS-COV-2 Spike glycoprotein, SARS-CoV-2, SARSCOV2 Spike protein, Severe Acute Respiratory Syndrome Coronavirus 2 Spike Protein, Spike, Spike glycoprotein, surface glycoprotein

Citations for Recombinant SARS-CoV-2 Spike (Mink Cluster-5) His Protein CF

Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.

Recombinant SARS-CoV-2 Spike (Mink Cluster-5) His Protein CF

Catalog # 10736-CV | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Product Specifications for Recombinant SARS-CoV-2 Spike (Mink Cluster-5) His Protein CF

Scientific Data Examples for Recombinant SARS-CoV-2 Spike (Mink Cluster-5) His Protein CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Spike

Citations for Recombinant SARS-CoV-2 Spike (Mink Cluster-5) His Protein CF

Product Documents for Recombinant SARS-CoV-2 Spike (Mink Cluster-5) His Protein CF

Product Datasheets

COA

Certificate of Analysis

SDS

Bio-Techne 品牌

保持关注