Recombinant SARS-CoV-2 B.1.640 Spike GCN4-IZ His Protein, CF

Name: Recombinant SARS-CoV-2 B.1.640 Spike GCN4-IZ His Protein, CF
Brand: R&D Systems
SKU: 11076-CV

Catalog # 11076-CV | R&D Systems, Inc. a Bio-Techne Brand

Product Datasheet / COA / SDS

His-tag

Setting the standard in quality research reagents

Key Product Details

Source:	HEK293
Accession #:	YP_009724390.1
Applications:	Bioactivity

View Full Technical Details for Recombinant SARS-CoV-2 B.1.640 Spike GCN4-IZ His Protein, CF

Technical Data
Citations / Reviews

Product Specifications for Recombinant SARS-CoV-2 B.1.640 Spike GCN4-IZ His Protein, CF

Source

Human embryonic kidney cell, HEK293-derived sars-cov-2 Spike protein

SARS-CoV-2 B.1.640 Spike (Val16-Lys1211) (Glu96Gln, Cys-Asn-Asp-Pro-Phe-Leu-Gly-Val-Tyr136-144del, Arg190Ser, Ile210Thr, Arg346Ser, Asn394Ser, Tyr449Asn, Phe490Arg, Asn501Tyr, Asp614Gly, Pro681His, Thr859Asn, Asp936His) (Arg682Ser, Arg685Ser, Lys986Pro, Val987Pro) Accession # YP_009724390.1	GCN4-IZ	6-His tag
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Val16

Predicted Molecular Mass

137 kDa

SDS-PAGE

137 kDa

Activity

Measured by its binding ability in a functional ELISA with Recombinant Human ACE-2 His-tag (Catalog # 933-ZN).

Scientific Data Examples for Recombinant SARS-CoV-2 B.1.640 Spike GCN4-IZ His Protein, CF

	Recombinant SARS-CoV-2 B.1.640 Spike (GCN4-IZ) His-tag Protein Binding Activity. Recombinant SARS-CoV-2 B.1.640 Spike (GCN4-IZ) His-tag Protein (Catalog # 11076-CV) binds Recombinant Human ACE-2 His-tag (933-ZN) in a functional ELISA.
	Recombinant SARS-CoV-2 B.1.640 Spike (GCN4-IZ) Histag Protein SDS-PAGE. 2 μg/lane of Recombinant SARS-CoV-2 B.1.640 Spike (GCN4-IZ) Histag Protein (Catalog # 11076-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 149-165 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11076-CV

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Spike

SARS-CoV-2, which causes the global pandemic coronavirus disease 2019 (Covid-19), belongs to a family of viruses known as coronaviruses that also include MERS and SARS-CoV-1. Coronaviruses are commonly comprised of four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M) and Nucleocapsid protein (N) (1). The SARS-CoV-2 S protein is a glycoprotein that mediates membrane fusion and viral entry. The S protein is homotrimeric, with each ~180-kDa monomer consisting of two subunits, S1 and S2 (2). In SARS-CoV-2, as with most coronaviruses, proteolytic cleavage of the S protein into S1 and S2 subunits is required for activation. The S1 subunit is focused on attachment of the protein to the host receptor while the S2 subunit is involved with cell fusion (3-5). A metallopeptidase, angiotensin-converting enzyme 2 (ACE-2), has been identified as a functional receptor for SARS-CoV-2 through interaction with a receptor binding domain (RBD) located at the C-terminus of S1 subunit (6,7). The S protein of SARS-CoV-2 shares 75% and 29% aa identity with the S protein of the SARS-CoV-1 and MERS. A SARS-CoV-2 variant (B.1.640 or B.1.640.1) carrying the aa substitution Arg346Ser, Asn394Ser, Tyr449Asn, Phe490Arg, and Asn501Tyr in the RBD was identified in samples from France, Indonesia, and Republic of the Congo (8). Whether these mutations in RBD would cause more severe symptom or decrease the efficacy of vaccine-induced immunity is still under investigation.

Item	Value
References	Wu, F. et al. (2020) Nature 579:265. Tortorici, M.A. and D. Veesler (2019). Adv. Virus Res. 105:93. Bosch, B.J. et al. (2003). J. Virol. 77:8801. Belouzard, S. et al. (2009) Proc. Natl. Acad. Sci. 106:5871. Millet, J.K. and G.R. Whittaker (2015) Virus Res. 202:120. Li, W. et al. (2003) Nature 426:450. Wong, S.K. et al. (2004) J. Biol. Chem. 279:3197. Colson, P. et al. (2021) medRxiv https://doi.org/10.1101/2021.12.24.21268174.
Long Name	Spike Protein
Entrez Gene IDs	918758 (HCoV-229E); 2943499 (HCoV-NL63); 39105218 (HCoV-OC43); 37616432 (MERS-CoV); 1489668 (SARS-CoV); 43740568 (SARS-CoV-2)
Alternate Names	2019-nCoV S Protein, 2019-nCoV Spike, COVID-19 Spike, E2, Human coronavirus spike glycoprotein, Peplomer protein, S Protein, S glycoprotein, SARS-COV-2 S protein, SARS-COV-2 Spike glycoprotein, SARS-CoV-2, SARSCOV2 Spike protein, Severe Acute Respiratory Syndrome Coronavirus 2 Spike Protein, Spike, Spike glycoprotein, surface glycoprotein

Citations for Recombinant SARS-CoV-2 B.1.640 Spike GCN4-IZ His Protein, CF

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Recombinant SARS-CoV-2 B.1.640 Spike GCN4-IZ His Protein, CF

Catalog # 11076-CV | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Product Specifications for Recombinant SARS-CoV-2 B.1.640 Spike GCN4-IZ His Protein, CF

Scientific Data Examples for Recombinant SARS-CoV-2 B.1.640 Spike GCN4-IZ His Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Spike

Citations for Recombinant SARS-CoV-2 B.1.640 Spike GCN4-IZ His Protein, CF

Product Documents for Recombinant SARS-CoV-2 B.1.640 Spike GCN4-IZ His Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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