Recombinant S. cerevisiae ATP Sulfurylase/MET3 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7175-AS

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
7175-AS-020
Print Quote
Bulk Order
100% Guarantee

Key Product Details

  • R&D Systems E. coli-derived Recombinant S. cerevisiae ATP Sulfurylase/MET3 Protein (7175-AS)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession #

P08536

Conjugate

Unconjugated

Applications

Enzyme Activity

View all ATP-Sulfurylase/MET3 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived yeast ATP-Sulfurylase/MET3 protein
Pro2-Phe511, with N-terminal Met and 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

59 kDa

SDS-PAGE

57-58 kDa, reducing conditions

Activity

Measured by its ability to sulfurylate ATP.
The specific activity is >2,500 pmol/min/μg, as measured under the described conditions.

Scientific Data Images for Recombinant S. cerevisiae ATP Sulfurylase/MET3 Protein, CF

Recombinant Yeast ATP‑Sulfurylase/MET3 SDS-PAGE.

2 μg/lane of Recombinant Yeast ATP‑Sulfurylase/MET3 (Catalog # 7175-AS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 57-58 kDa.

Formulation, Preparation and Storage

7175-AS
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, Glycerol and DTT.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: ATP-Sulfurylase/MET3

Sulfur metabolism is essential to all organisms. Inorganic sulfate is sequentially activated by ATP Sulfurylase and adenosine 5′-phosphosulfate kinase (APSK). ATP Sulfurylase converts ATP to adenosine-5'-phosphosulfate (APS) and generates pyrophosphate (1). APSK subsequently phosphorylates APS at the 3’ site to generate 3'‑phosphoadenosine-5'-phosphosulfate (PAPS) (2). PAPS is the sulfur donor required for all sulfotransferase reactions in humans and is also the sulfur source for synthesis of cysteine, methionine and glutathione (3). The ATP Sulfurylase reaction is highly unfavorable energetically with the standard free-energy change of approximately +46 kJ/mol (4). To drive the reaction forward, APSK and pyrophosphatase are generally added into the reaction simultaneously to remove the products. The recombinant S. cerevisiae ATP Sulfurylase can be used for in vitro PAPS synthesis (5, 6).

References

  1. Robbins, P. W. and Lipmann, F. (1958) J. Biol. Chem. 233:686.
  2. MacRae, I.J. et al. (1998) J. Biol. Chem. 273:28583.
  3. Strott, C.A. (2002) Endocr. Rev. 23:703.
  4. Hawes, C.S. and Nicholas, D.J.D. (1973) Biochem. J. 133: 541.
  5. Wu, Z.L. et al. (2002) The FASEB J. 16:539.
  6. Lin, C.H. et al. (1995) J. Am.Chem. Soc. 117:8031.

Alternate Names

ATPSulfurylase, MET3

Entrez Gene IDs

853466 (Yeast)

Gene Symbol

MET3

UniProt

P08536

Additional ATP-Sulfurylase/MET3 Products

Product Documents for Recombinant S. cerevisiae ATP Sulfurylase/MET3 Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant S. cerevisiae ATP Sulfurylase/MET3 Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.