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Recombinant Rat BMP-6 Protein, CF

Catalog # 10420-BM | R&D Systems, Inc. a Bio-Techne Brand
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10420-BM-020

Key Product Details

Source

CHO

Accession #

Structure / Form

Covalent dimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived rat BMP-6 protein
Ser368-His506

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser368

Predicted Molecular Mass

16 kDa

SDS-PAGE

16-22 kDa, under reducing conditions

Activity

Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351.
The ED50 for this effect is 0.15-0.9 μg/mL.

Scientific Data Images for Recombinant Rat BMP-6 Protein, CF

Recombinant Rat BMP-6 Protein Bioactivity

Recombinant Rat BMP-6 Protein Bioactivity

Recombinant Rat BMP-6 (Catalog # 10420-BM) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The ED50 for this effect is 0.15-0.9 μg/mL.
Recombinant Rat BMP-6 Protein SDS-PAGE

Recombinant Rat BMP-6 Protein SDS-PAGE

2 μg/lane of Recombinant Rat BMP-6 (Catalog # 10420-BM) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 16-22 kDa and 32-44 kDa, respectively.

Formulation, Preparation and Storage

10420-BM
Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution Reconstitute at 200 μg/mL in 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-6

Bone Morphogenetic Protein 6 (BMP-6), also known as Vgr-1, is a member of the BMP subfamily of TGF-beta superfamily proteins. BMPs are involved in a wide range of processes including embryogenesis, tissue morphogenesis, cell differentiation and migration, and tumorigenesis (1). BMP-6 is synthesized as a large precursor protein that is cleaved at the dibasic cleavage site (RxxR) to release a smaller, C-terminal mature protein. Biologically active BMP-6 consists of a disulfide-linked homodimer of the mature protein, with each monomer containing a characteristic cystine knot motif (2). Mature rat BMP-6 shares 98% and 96% amino acid sequence identity with human and mouse BMP-6, respectively. Cellular responses to BMP-6 are mediated by hetero-oligomeric complexes of type I (Activin RIA/ALK-2 and BMPR-IA/ALK-3) and type II (Activin RIIA and BMPR-II) serine/threonine kinase receptors (3-5). BMP-6 induces the expression of Noggin and is subsequently antagonized by Noggin (6). BMP-6 induces a wide range of cellular responses. It promotes osteoblast differentiation from mesenchymal stem cells (7), chondrocyte maturation (8), Ang II-induced aldosterone production in the adrenal cortex (4), hormone production and responsiveness in ovarian granulosa cells (9), iNOS and TNF-alpha production in macrophages (5), the cell death of B cells (10), and neurite outgrowth (11). BMP-6 expression is induced in astrocytes surrounding sites of brain injury where it functions as a neuroprotectant (11, 12). It enhances tumor progression by promoting local angiogenesis and differentiation of immune tolerizing M2 macrophages (13-15). Through interactions with the BMP co-receptor RGM-C/Hemojuvelin, BMP-6 plays an important role in iron homeostasis by promoting Hepcidin expression and preventing serum iron overload (16). Heterodimers of BMP-2 and BMP-6 show increased potency at inducing osteoblastic calcium deposition, chondrogenesis, and in vivo bone formation compared to either BMP-2 or BMP-6 homodimers (3).

References

  1. Bragdon, B. et al. (2010) Cell Signal. 23:609.
  2. Allendorph GP et al. (2007) Biochemistry. 46:12238.
  3. Israel, D.I. et al. (1996) Growth Factors 13:291.
  4. Inagaki, K. et al. (2006) Endocrinology 147:2681.
  5. Hong, J.H. et al. (2008) Immunology 128:e442.
  6. Haudenschild, D.R. et al. (2004) Cancer Res. 64:8276.
  7. Lavery, K. et al. (2008) J. Biol. Chem. 283:20948.
  8. Grimsrud, C.D. et al. (1999) J. Bone Miner. Res. 14:475.
  9. Shi, J. et al. (2009) Fertil. Steril. 92:1794.
  10. Kersten, C. et al. (2005) BMC Immunol. 6:9.
  11. Yabe, T. et al. (2002) J. Neurosci. Res. 68:161.
  12. Zhang, Z. et al. (2006) Neuroscience 138:47.
  13. Dai, J. et al. (2005) Cancer Res. 65:8274.
  14. Kwon, S.J. et al. (2014) Prostate 74:121.
  15. Lee, J.-H. et al. (2013) Cancer Res. 73:3604.
  16. Andriopoulos, B. Jr. et al. (2009) Nat. Genet. 41:482.

Long Name

Bone Morphogenetic Protein 6

Alternate Names

BMP6, Vgr-1

Entrez Gene IDs

654 (Human); 12161 (Mouse); 25644 (Rat)

Gene Symbol

BMP6

UniProt

Product Documents for Recombinant Rat BMP-6 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Rat BMP-6 Protein, CF

For research use only

FAQs for Recombinant Rat BMP-6 Protein, CF

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  • Q: What is the half-life of a recombinant or natural protein in culture at 37 °C?

    A: R&D Systems has not determined the half-life of recombinant or natural proteins in culture. For cell-based bioassays, the incubation period of the protein with the cells typically ranges from 48 to 96 hours. Assay conditions for each protein will need to be optimized by each researcher.

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